Antiproliferative activity of methanolic extracts from two green algae, Enteromorpha intestinalis and Rizoclonium riparium on HeLa cells

Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences
Subhabrata Paul, Rita Kundu

Abstract

Natural compounds can be alternative sources for finding new lead anti-cancer molecules. Marine algae have been a traditional source for bioactive compounds. Enteromorpha intestinalis and Rhizoclonium riparium are two well distributed saline/brackish water algae from Sundarbans. There's no previous report of these two for their anti-proliferative activities. Cytotoxicity of the algal methanolic extracts (AMEs) on HeLa cells were assayed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) reduction assay. Morphological examinations were done by Haematoxylin, Hoechst 33258 and Acridine orange staining. DNA fragmentation was checked. Gene expressions of Cysteine aspartate protease (Caspase) 3, Tumor protein (TP) 53, Bcl-2 associated protein X (Bax) were studied by Reverse transcription- polymerase chain reaction (RT-PCR) keeping Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as internal control. Protein expressions were studied for Caspase 3, phospho-p53, Bax, Microtubule associated proteins-1/ light chain B (MAP1/LC3B) by western blot. The AMEs were found to be cytotoxic with Inhibitory concentration 50 (IC50) values 309.048 ± 3.083 μg/ml and 506.081 ± 3.714 μg/ml for E. intestinalis and R. riparium extract...Continue Reading

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Citations

Sep 26, 2014·Marine Drugs·Ghislain MoussavouYoung Kug Choo
Mar 10, 2016·Pharmaceutical Biology·Idalia Osuna-RuizMiguel A Hurtado-Oliva
Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hadeer A AglanNadia S Mahmoud
Jul 28, 2016·Marine Drugs·Nadia RuoccoMaria Costantini
Nov 27, 2020·Molecular Biology Reports·Biswajita PradhanMrutyunjay Jena

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Methods Mentioned

BETA
electrophoresis
transfection
PCRs
PCR

Software Mentioned

Primer
UVP Multidoc - It
Primer3
UVP Doc - ItLS
Graphpad Prism

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