Antiproliferative and Anti-invasion Effects of Carvacrol on PC3 Human Prostate Cancer Cells through Reducing pSTAT3, pAKT, and pERK1/2 Signaling Proteins

International Journal of Preventive Medicine
Esfandiar Heidarian, Mahnaz Keloushadi

Abstract

One of the most effective parameters in the progression of the prostate cancer is interleukin (IL)-6 through affecting pSTAT3, pERK1/2, and pAKT cell signaling proteins. Carvacrol is an herbal antioxidant with antitumor effects. The purpose of this study was to investigate the effects of carvacrol on IL-6 gene expression, pSTAT3, pAKT, pERK1/2 cellular signaling proteins, and invasion in human prostate cancer PC3 cells. PC3 cell viability was evaluated by MTT assay with different concentrations of carvacrol (0-800 μM). IL-6 gene expression and cellular concentration of pSTAT3, pERK1/2, and pAKT were investigated using the real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blotting technic, respectively. PC3 cell invasion was determined by invasion assay test. Carvacrol IC50 for PC3 prostate cancer cells was 360 μM. Carvacrol led to a significant reduction (P < 0.05) for IL-6 gene expression in a dose-dependent manner compared to control. IL-6 protein reduced 41.5% and 52.7% when compared with control cells at 360 and 420 μM of carvacrol, respectively. Carvacrol led to a decline in pSTAT3, pAKT, and pERK1/2 above 360 μM compared to control. PC3 potential invasion was significantly reduce...Continue Reading

References

Sep 27, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Josep Domingo-DomenechBegoña Mellado
Jun 18, 2010·European Journal of Pharmacology·Yong LuShing-Shun Tony To
Feb 5, 2013·Asian Pacific Journal of Tropical Medicine·Balakrishnan AristatileKodukkur Viswanathan Pugalendi
Sep 24, 2013·BJU International·Daniel P NguyenAshutosh K Tewari
Dec 12, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·María Llana-Ruiz-CabelloAna María Cameán
Jan 15, 2014·Journal of Dietary Supplements·Esfandiar HeidarianEffat Jafari-Dehkordi
Feb 21, 2014·Seminars in Immunology·Koji Taniguchi, Michael Karin
Mar 10, 2015·Expert Opinion on Investigational Drugs·Matteo SantoniStefano Cascinu
Apr 2, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yuting CuiXuebo Liu
Sep 26, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Esfandiar HeidarianParisa Valipour

❮ Previous
Next ❯

Citations

Feb 23, 2020·Seminars in Cancer Biology·Sajad FakhriAnupam Bishayee
Jul 27, 2021·Frontiers in Pharmacology·Laeza Alves SampaioAdriana Gibara Guimarães

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Software Mentioned

SPSS
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.