Antiproliferative and antitumorigenic activity of Toona sinensis leaf extracts in lung adenocarcinoma

Journal of Medicinal Food
Chih-Jen YangChing-Feng Weng

Abstract

Toona sinensis is a traditional Chinese herb, and the extracts of T. sinensis leaf possess a variety of biological functions. This study attempted to test the antiproliferative effect of TSL-1 (a bioactive fraction of T. sinensis) in H441 cells (lung adenocarcinoma). The data showed that the antiproliferative effect of TSL-1 on H441 cells is prominent using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. TSL-1-induced apoptosis was confirmed by cell morphology, sub-G(1) peak accumulation, cleavage of poly(ADP)-ribose polymerase, and propidium iodide-annexin V double staining. Furthermore, decreased Bcl-2 accompanied by increased Bax (in western blotting) was found with TSL-1 treatment of H441 cells. TSL-1 treatment-induced G(1) arrest was concurrent with the down-regulation of protein levels of cyclin D1 and cyclin-dependent kinase 4 in H441 cells. Peroral and intraperitoneal administrations of TSL-1 were performed to evaluate the therapeutic efficacy, and peroral administration of TSL-1 was also used to elucidate the therapeutic efficacy in the H441 cell xenograft model in vivo. The data revealed that TSL-1 treatment inhibited H441 tumor growth in both therapeutic and preventive experiments. Taken toget...Continue Reading

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Citations

Jan 22, 2014·The Kaohsiung Journal of Medical Sciences·Chao-Chuan WangChih-Lung Lin
Jun 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jie ShenMingKang Zhong
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Jul 24, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jyoti SinghAbha Meena

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Methods Mentioned

BETA
fluorescence-activated
flow cytometry
protein assay
electrophoresis
xenograft

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