Antiproliferative and apoptotic effect of LY2090314, a GSK-3 inhibitor, in neuroblastoma in vitro

BMC Cancer
Selvi KunnimalaiyaanMuthusamy Kunnimalaiyaan

Abstract

Neuroblastoma (NB) is a devastating disease. Despite recent advances in the treatment of NB, about 60% of high-risk NB will have relapse and therefore long-term event free survival is very minimal. We have reported that targeting glycogen synthase kinase-3 (GSK-3) may be a potential strategy to treat NB. Consequently, investigating LY2090314, a clinically relevant GSK-3 inhibitor, on NB cellular proliferation and may be beneficial for NB treatment. The effect of LY2090314 was compared with a previously studied GSK-3 inhibitor, Tideglusib. Colorimetric, clonogenic, and live-cell image confluency assays were used to study the proliferative effect of LY2090314 on NB cell lines (NGP, SK-N-AS, and SH-SY-5Y). Western blotting and caspase glo assay were performed to determine the mechanistic function of LY2090314 in NB cell lines. LY2090314 treatment exhibited significant growth reduction starting at a 20 nM concentration in NGP, SK-N-AS, and SH-SY-5Y cells. Western blot analysis indicated that growth suppression was due to apoptosis as evidenced by an increase in pro-apoptotic markers cleaved PARP and cleaved caspase-3 and a reduction in the anti-apoptotic protein, survivin. Further, treatment significantly reduced the level of cycli...Continue Reading

References

Oct 31, 1985·The New England Journal of Medicine·R C SeegerD Hammond
Aug 4, 2004·Seminars in Cancer Biology·Sven PåhlmanHåkan Axelson
Mar 25, 2006·Future Oncology·Andrei V Ougolkov, Daniel D Billadeau
Jun 26, 2007·Lancet·John M MarisSusan L Cohn
Dec 3, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Susan L CohnUNKNOWN INRG Task Force
Jun 19, 2010·The New England Journal of Medicine·John M Maris
Aug 19, 2010·Neuro-oncology·Giulia Ferrari-ToninelliMaurizio Memo
Jul 12, 2011·Pediatric Blood & Cancer·Peter E ZagePatrick A Zweidler-McKay
Nov 7, 2012·Journal of Pediatric Hematology/oncology·Sule AylaGulperi Oktem
Oct 3, 2013·Cold Spring Harbor Perspectives in Medicine·Miller Huang, William A Weiss
Oct 31, 2013·Advances in Biological Regulation·James A McCubreyLinda S Steelman
Feb 14, 2014·Cancer Biology & Therapy·Yvette M CarterMuthusamy Kunnimalaiyaan
Jun 17, 2014·Oncotarget·James A McCubreyMelchiorre Cervello
Aug 17, 2014·The Journal of Surgical Research·Jessica A NaiditchMary Beth Madonna
Apr 19, 2015·Molecular Cancer Therapeutics·Selvi KunnimalaiyaanMuthusamy Kunnimalaiyaan
Aug 5, 2016·Environmental Toxicology and Pharmacology·Theodore Lemuel MathuramKotturathu Mammen Cherian
Jan 6, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amy WalzAndrew P Mazar
Nov 21, 2017·Oncotarget·Daniel WaldenMuthusamy Kunnimalaiyaan

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Citations

Sep 26, 2019·Proceedings of the National Academy of Sciences of the United States of America·Paul B RomesserScott W Lowe
Feb 6, 2020·Life Science Alliance·Alessio ReggioFrancesca Sacco
Apr 22, 2020·Heart Failure Reviews·Arun K SharmaHanan Hagar
May 10, 2020·Cell Death and Differentiation·Alessio ReggioGianni Cesareni
Jun 14, 2019·Frontiers in Molecular Neuroscience·Hisham F BahmadWassim Abou-Kheir
Nov 13, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tomoko SugiuraTakeshi Urano
Jan 29, 2019·Journal of the American Chemical Society·Fengfei WangErxi Wu
Dec 1, 2020·Frontiers in Neuroscience·Xu YanXinwen Zhang
Nov 7, 2020·Medicinal Research Reviews·Atif ZafarRuiwen Zhang
Aug 31, 2021·Signal Transduction and Targeted Therapy·Fanyuan YuLing Ye

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Methods Mentioned

BETA
protein assay
imaging techniques

Software Mentioned

IncuCyte
Image Lab
SPSS Statistics

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