PMID: 18202806Jan 19, 2008Paper

Antiproliferative and proapoptotic effects of rapamycin and celecoxib in malignant melanoma cell lines

Oncology Reports
Annika BundschererThomas Vogt

Abstract

Inhibitors of cyclooxygenase 2 (COX 2) and the mammalian target of rapamycin (mTOR) show direct and indirect antitumor effects in a variety of cancers. This study was designed to investigate the effects of the mTOR antagonist rapamycin and the COX 2 inhibitor celecoxib on cell growth and apoptosis in malignant melanoma. Cell proliferation was analysed by the cell proliferation ELISA BrdU and alamarBlue assay and apoptosis was measured by caspase 3 and 7 activity in two out of six melanoma cell lines (A375 and Mel Ho) that were selected for the heterogeneous levels of the COX 2 mRNA expression. The quantitative real-time reverse transcription polymerase chain reaction showed a 337-fold higher COX 2 mRNA level in the A375 than in the Mel Ho melanoma cells. However, both celecoxib and rapamycin caused significant growth inhibition in the two cell lines. By combining both agents, additive growth inhibitory effects were observed in the A375 cells. Treatment with celecoxib, but not rapamycin, increased apoptosis in the two cell lines. Our data indicate that rapamycin and celecoxib inhibit melanoma cell growth as single agents and a combination of both drugs have additive antitumor effects. Notably, the antiproliferative and proapopto...Continue Reading

Citations

Mar 4, 2014·Der Anaesthesist·A BundschererB M Graf
Nov 21, 2009·Journal of the American Academy of Dermatology·Vivian LaquerKristen M Kelly
Jul 10, 2009·The Journal of Investigative Dermatology·Arjen JoosseTamar Nijsten
Mar 25, 2014·Research in Veterinary Science·Kyoung-Won SeoHwa-Young Youn
Jun 21, 2012·Anais Brasileiros De Dermatologia·Juliano Vilaverde Schmitt, Hélio Amante Miot
Jun 12, 2014·Revista da Associação Médica Brasileira·Juliano Schmitt, Hélio Miot

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