Antiproliferative effect of isolated isoquinoline alkaloid from Mucuna pruriens seeds in hepatic carcinoma cells

Natural Product Research
Pranesh KumarSudipta Saha

Abstract

The present study was undertaken to investigate the antiproliferative action of isolated M1 (6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) from Mucuna pruriens seeds using human hepatic carcinoma cell line (Huh-7 cells). Initially, docking studies was performed to find out the binding affinities of M1 to caspase-3 and 8 enzymes. Later, cytotoxic action of M1 was measured by cell growth inhibition (MTT), followed by caspase-3 and 8 enzymes assay colorimetrically. Our results collectively suggested that M1 had strong binding affinity to caspase-8 in molecular modelling. M1 possessed antiproliferative activity on Huh-7 cells (EC50 = 13.97 μM) and also inhibited the action of caspase-8 enzyme, signified process of apoptosis. M1 was active against Huh-7 cells that may be useful for future hepatic cancer treatment.

References

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Citations

May 11, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kuen-Bao ChenYu-Chian Chen
Jul 31, 2020·Medicinal Research Reviews·Xiao-Fei ShangKuo-Hsiung Lee
Oct 16, 2016·Applied Microbiology and Biotechnology·Salwa Mansur AliNaveed Ahmed Khan
Feb 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Priyanka MishraSudipta Saha
Aug 15, 2021·Journal of Ethnopharmacology·Emmanuel Ayodeji AyeniXun Liao

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Methods Mentioned

BETA
caspase assay

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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