Antiproliferative effects of anastrozole on MCF-7 human breast cancer cells in vitro are significantly enhanced by combined treatment with testosterone undecanoate

Molecular Medicine Reports
Rong ChenWei Wei

Abstract

The present study aimed to assess the effects of aromatase inhibitor anastrozole and testosterone undecanoate, separately and in combination, on proliferation and apoptosis in MCF-7 human breast cancer cells cultured in vitro. The effects of various concentrations of these drugs on the proliferation of MCF-7 cells were evaluated by CCK8 assay, the levels of cell apoptosis were evaluated by flow cytometry with Annexin-V/propidium iodide staining and androgen receptor (AR) protein expression was determined by western blot analysis. The results of the CCK8 assay indicated that greater antiproliferative activity was detected in the MCF-7 cells in the combined treatment groups, compared with those treated with anastrozole or testosterone undecanoate alone. Flow cytometric analysis of apoptosis revealed that treatment with a combination of the two drugs generated a higher percentage of apoptotic cells, particularly when the two drugs were applied for 48 h, compared with single drug treatment. Western blot analysis revealed a significant decrease in AR protein expression in the combined treatment groups compared with MCF7 cells treated with single drugs. The results of the present study provided evidence supporting the potential of a ...Continue Reading

References

Oct 21, 1992·International Journal of Cancer. Journal International Du Cancer·V Kuenen-BoumeesterS C Henzen-Logmans
Jun 8, 2004·Endocrine Reviews·Woraluk SomboonpornUNKNOWN National Health and Medical Research Council
Sep 10, 2004·Menopause : the Journal of the North American Menopause Society·Constantine DimitrakakisCarolyn A Bondy
Dec 3, 2005·Endocrine-related Cancer·Nicola NormannoUNKNOWN NCI-Naples Breast Cancer Group
Feb 11, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Judith HughCharles Vogel
Jul 30, 2009·Pharmacotherapy·Serena T Wong, Susan Goodin
Nov 10, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Leo A NiemeierRohit Bhargava
Feb 4, 2010·Breast Cancer Research and Treatment·Isabella CastellanoAnna Sapino
Feb 26, 2010·The Lancet Oncology·Britta WeigeltJorge S Reis-Filho
Jul 14, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Harold J BursteinUNKNOWN American Society of Clinical Oncology
Nov 27, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·Q YuX Q Xiao
Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rong HuRulla M Tamimi
Feb 26, 2011·Endocrine, Metabolic & Immune Disorders Drug Targets·Giorgio Santoni, Valerio Farfariello
Jun 28, 2011·The EMBO Journal·Jessica L L RobinsonJason S Carroll
Apr 11, 2013·Breast Cancer Research and Treatment·C CampagnoliF Berrino
Apr 30, 2013·Endocrine-related Cancer·Per Eystein Lønning, Hans Petter Eikesdal

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Citations

Mar 6, 2016·Current Opinion in Endocrinology, Diabetes, and Obesity·Keely M McNamara, Hironobu Sasano
Jan 1, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Cristina AmaralNatércia Teixeira
Mar 17, 2021·Post Reproductive Health·A ScottD Bruce

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis