Antiproteolytic action of orally delivered insulin using pH-responsive hydrogels in a rat burn model

The Journal of Surgical Research
Sundararajan MadihallyMehmet Toner

Abstract

Subcutaneously delivered small doses of insulin has shown beneficial effects on burn injury-induced muscle wasting and wound healing. To improve the method of insulin treatment for clinical settings, this study investigated the effect of insulin delivered orally using pH-responsive poly(methacrylic-g-ethylene glycol) (P(MAA-g-EG)) hydrogels in a 20% total burn surface area rat burn injury model. P(MAA-g-EG) were synthesized in-house and insulin release characteristics were performed in vitro. Young rats weighing 80-150 g were subjected to 15-20% total body surface area burn injury and treated with insulin-containing hydrogels enclosed in gelatin capsules for 3 days. The dosage was adjusted to match 0.25 U (day 1), 0.5U (day 2), and 1.0 U (day 3) per 100 grams of body weight. All animals were housed in metabolic cages and their physical activity, body weight, food consumption, water uptake, circulating glucose levels, and urinary tyrosine content were monitored for 4 to 15 days after burn. Results show that the orally delivered insulin restored the body weight of burned rats and influenced wound healing, similar to subcutaneous delivery. Measured glucose levels showed significantly less perturbation, suggesting the possibility o...Continue Reading

References

Dec 1, 1988·Diabetes/metabolism Reviews·I C KettelhutA L Goldberg
Aug 1, 1986·Metabolism: Clinical and Experimental·R Odessey
May 1, 1986·Clinical Endocrinology·K N Frayn
Jan 1, 1994·Annual Review of Physiology·S R KimballL S Jefferson
Jan 1, 1997·Annual Review of Nutrition·O E Rooyackers, K S Nair
Mar 14, 1998·The Journal of Trauma·E J PierreD N Herndon
Jun 2, 1998·The British Journal of Surgery·M Schäffer, A Barbul
Aug 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·P O Hasselgren
Sep 10, 1999·Journal of Pharmaceutical Sciences·A M LowmanN A Peppas
May 7, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mariko MorishitaNicholas A Peppas
Jul 18, 2002·The Journal of Surgical Research·Vered SolomonMehmet Toner
Dec 24, 2002·Annals of the New York Academy of Sciences·Marie OshimaKiyoshi Takagi
Mar 20, 2003·The Journal of Surgical Research·Sundararajan V MadiballyMehmet Toner
Mar 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Koji NakamuraAnthony M Lowman
May 19, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mariko MorishitaAnthony M Lowman
Mar 14, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Xiao-jun ZhangRobert R Wolfe

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