Antiquorum sensing, antibiofilm formation and cytotoxicity activity of commonly used medicinal plants by inhabitants of Borabu sub-county, Nyamira County, Kenya

PloS One
E O OmwengaF M Goycoolea

Abstract

Productions of various bacterial traits like production of virulence factors (e.g. toxins, enzymes), biofilm formation, luminescence among others, have been known to be controlled by quorum sensing (QS), a process that is dependent on chemical signals or autoinducers (AIs). Bacteria known to rely on such AIs are known to be virulent and tend to be resistant against various antimicrobial agents. Therefore, strategies aimed at the inhibition of QS pathways, are regarded as potential novel therapies in managing bacterial virulence hence reducing their ability to induce infections in humans. In the present study, a portfolio of 25 medicinal plant extracts (ethanol 50% v/v) used in southwestern Kenya were assayed against a transformed E. coli Top 10 reporter QS strain. This biosensor responds to the exogenous addition of 3-oxo-N-hexanoyl homoserine lactone (3OC6HSL) expressing green fluorescent protein (GFP). The large majority of the screened medicinal plants seemed to exhibit toxic effects and almost none of them induced antiquorum sensing (AQS) activity. This could be the consequence of the presence of mixed compounds in the extracts. Elaeodendron buchananii Loes and Acacia gerrardii Benth extracts that seemed to show AQS activit...Continue Reading

References

May 8, 2007·Annual Review of Microbiology·Rita TamayoAndrew Camilli
Jan 1, 2007·African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM·P G KareruG M Kenji
Aug 2, 2012·Antimicrobial Agents and Chemotherapy·Karthik SambanthamoorthyChristopher M Waters

❮ Previous
Next ❯

Citations

Apr 26, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Lekshmi NarendrakumarSabu Thomas
Nov 25, 2020·Antibiotics·Christian Emmanuel MahavyTsiry Rasamiravaka

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensor
environmental stress

Software Mentioned

Graph Pad
Graph Pad Prism

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.