Antiretroviral genotypic resistance in plasma RNA and whole blood DNA in HIV-1 infected patients failing HAART

Journal of Medical Virology
Annalisa SaracinoMutations, Salvage Study Group

Abstract

The extent to which HIV-1 proviral DNA mutations cause clinically relevant antiretroviral resistance is still controversial. Paired plasma HIV-1 RNA and whole blood DNA were compared in patients failing HAART to investigate if the additional knowledge of archived mutations could improve the selection of potentially active drugs. Seventy-three HIV-1-infected patients with first/second HAART failure were studied before starting a new regimen based on RNA genotyping. Follow-up data after a 12-week therapy were available. DNA genotyping was retrospectively performed on stored whole blood samples and mutational profiles were compared to those from RNA. The mean number of IAS pol mutations was significantly higher in RNA (4.45 +/- 2.76) than in DNA (2.88 +/- 2.47) (P < 0.001). DNA genotyping provided a 6% increase in detection of resistance-associated mutations. Among 64/73 patients showing discordant DNA/RNA profiles, 54 (84%) also differed for predicted active drugs. 16/73 (22%) patients had >or=1 mutation revealed by DNA genotyping alone, probably affecting therapy success in 2/16. However, neither RNA/DNA discordance nor detection of isolated DNA mutations were statistically associated with outcome. In conclusion, plasma RNA rema...Continue Reading

References

Feb 8, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·P Keiser
Mar 12, 2003·Journal of Medical Virology·Laura MonnoGioacchino Angarano
May 25, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Choo Beng ChewNitin K Saksena
Jun 17, 2005·AIDS·Mauro ZaccarelliUNKNOWN Collaborative Group for Clinical Use of HIV Genotype Resistance Test (GRT) at National Institute for Infectious Diseases Laz
Aug 19, 2005·The Journal of Infectious Diseases·Annemarie M J WensingUNKNOWN SPREAD Programme
Apr 19, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nicola GianottiUNKNOWN Mutations and Salvage (MuSa) Study Group
Dec 15, 2006·Journal of Clinical Microbiology·Amanda McNultyJ Gerardo García-Lerma
Jan 17, 2007·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Ombretta TurrizianiGuido Antonelli
Feb 20, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Isabella BonMaria Carla Re
Apr 24, 2007·The Journal of Antimicrobial Chemotherapy·Clare L Booth, Anna Maria Geretti
May 23, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ilaria VicentiMaurizio Zazzi

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Citations

Oct 13, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Rebekah L BenishRajeev K Mehlotra
Aug 3, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·O TurrizianiG Antonelli
Oct 5, 2018·PloS One·Kahsay HuruyMelanie Maier

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