Antiretroviral therapy-induced functional modification of IgG4 and IgM responses in HIV-1-infected individuals screened by an allosteric biosensor

Journal of Biomolecular Screening
Rosa Maria FerrazAntonio Villaverde

Abstract

We have explored the effect of antiretroviral drugs on the antiviral immune response in human immunodeficiency virus-1 (HIV-1)-infected patients by using an enzymatic immunosensor that detects epitope-modifying anti-gp41 antibodies. By this molecular sensing approach, we have identified an irreversible impact of drug administration on the functionality of IgG4 and IgM specific antibodies regarding the structural modification promoted on their target epitope. During the antiretroviral therapy, the prevalent induced fit promoted by IgM on the epitope was lost at the expense of that promoted by IgG4, suggesting alternative-ness in the neutralization potency of these antibody subpopulations. Because the particular drug composition of the antiretroviral treatment did not affect such immune shift, the obtained data strongly suggest that the drop in the viral load and the consequent lost of antigenemia are responsible for the functional adaptation observed in the humoral response.

References

May 1, 1996·Immunology Today·P PoignardQ J Sattentau
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·D G MyszkaM L Doyle
Aug 2, 2001·The Journal of Biological Chemistry·N Ferrer-MirallesA Villaverde
Mar 21, 2003·Nature·Xiping WeiGeorge M Shaw
Feb 9, 2006·Molecular Immunology·Rosa María FerrazAntonio Villaverde
Apr 6, 2006·Microbial Cell Factories·Rosa María FerrazAntonio Villaverde
Jan 1, 1972·Annales de génétique et de sélection animale·J Quisenberry

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Citations

Jul 22, 2011·Veterinary Medicine International·Neeta LongjamV K Saxena
Jan 30, 2009·Journal of Molecular Recognition : JMR·Rosa María FerrazAntonio Villaverde
Apr 26, 2011·Biotechnology Advances·Carla OliveiraLucília Domingues
Jun 26, 2009·Clinical and Vaccine Immunology : CVI·M Teresa Sánchez-AparicioFrancisco Sobrino

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