PMID: 9525588Apr 3, 1998Paper

Antirotavirus immunoglobulin A neutralizes virus in vitro after transcytosis through epithelial cells and protects infant mice from diarrhea

Journal of Virology
F M RuggeriL Svensson

Abstract

Rotaviruses are the major cause of severe diarrhea in infants and young children worldwide. Due to their restricted site of replication, i.e., mature enterocytes, local intestinal antibodies have been proposed to play a major role in protective immunity. Whether secretory immunoglobulin A (IgA) antibodies alone can provide protection against rotavirus diarrhea has not been fully established. To address this question, a library of IgA monoclonal antibodies (MAbs) previously developed against different proteins of rhesus rotavirus was used. A murine hybridoma "backpack tumor" model was established to examine if a single MAb secreted onto mucosal surfaces via the normal epithelial transport pathway was capable of protecting mice against diarrhea upon oral challenge with rotavirus. Of several IgA and IgG MAbs directed against VP8 and VP6 of rotavirus, only IgA VP8 MAbs (four of four) were found to protect newborn mice from diarrhea. An IgG MAb recognizing the same epitope as one of the IgA MAbs tested failed to protect mice from diarrhea. We also investigated if antibodies could be transcytosed in a biologically active form from the basolateral domain to the apical domain through filter-grown Madin-Darby canine kidney (MDCK) cells ...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M B MazanecJ G Nedrud
Oct 1, 1992·Physiological Reviews·J P Kraehenbuhl, M R Neutra
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C S KaetzelM E Lamm
Apr 1, 1991·Journal of Virology·K B Renegar, P A Small
Oct 15, 1990·The Biochemical Journal·M A Kerr
Dec 1, 1986·Virology·R D ShawH B Greenberg
Jan 1, 1989·Advances in Virus Research·S M MatsuiH B Greenberg
Jan 1, 1989·Annual Review of Microbiology·N K ChildersJ R McGhee
Jun 1, 1988·The Pediatric Infectious Disease Journal·G A LosonskyM M Levine
Apr 1, 1988·Immunology and Cell Biology·I UhnooP L Ogra
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y HoshinoR M Chanock
Aug 1, 1987·Journal of Virology·M B MazanecM E Lamm
Nov 1, 1988·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J Mestecky
May 1, 1983·Infection and Immunity·G P DavidsonC P Kirubakaran
Feb 20, 1995·Virology·J W BurnsH B Greenberg
Mar 1, 1993·The Journal of Infectious Diseases·D O MatsonM K Estes

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Citations

Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·J BerdozB Corthésy
May 3, 2013·The Journal of Clinical Investigation·Emily M DealHarry B Greenberg
Nov 27, 2014·Human Vaccines & Immunotherapeutics·Suvi LappalainenVesna Blazevic
Oct 7, 2006·Current Opinion in Gastroenterology·R D Shaw
Jul 8, 2011·Journal of Pediatric Gastroenterology and Nutrition·Naflesia B O CorrêaLuciano A P Filho
Dec 3, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jerry M WellsClara L Garcia-Rodenas
Jun 20, 2013·Human Vaccines & Immunotherapeutics·Suvi LappalainenVesna Blazevic
Aug 9, 2013·The Journal of Clinical Investigation·Daisuke TokuharaYoshikazu Yuki
Mar 7, 2000·Clinical and Diagnostic Laboratory Immunology·Y Al-YousifS Kapil
Aug 10, 2010·Nature Reviews. Microbiology·Richard A Strugnell, Odilia L C Wijburg
Apr 9, 2001·Clinical Microbiology Reviews·P L OgraR C Welliver
Mar 26, 2003·The Journal of General Virology·Ali MirazimiLennart Svensson
Jul 28, 2016·PloS One·Sonja BialowasLennart Svensson
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C DevitoJ Hinkula
Sep 22, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·F X Lü
Mar 13, 2001·Journal of Molecular Biology·E ThouveninE Hewat

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