Antisense inhibition of the photosynthetic antenna proteins CP29 and CP26: implications for the mechanism of protective energy dissipation

The Plant Cell
J AnderssonS Jansson

Abstract

The specific roles of the chlorophyll a/b binding proteins CP29 and CP26 in light harvesting and energy dissipation within the photosynthetic apparatus have been investigated. Arabidopsis was transformed with antisense constructs against the genes encoding the CP29 or CP26 apoprotein, which gave rise to several transgenic lines with remarkably low amounts of the antisense target proteins. The decrease in the level of CP24 protein in the CP29 antisense lines indicates a physical interaction between these complexes. Analysis of chlorophyll fluorescence showed that removal of the proteins affected photosystem II function, probably as a result of changes in the organization of the light-harvesting antenna. However, whole plant measurements showed that overall photosynthetic rates were similar to those in the wild type. Both antisense lines were capable of the qE type of nonphotochemical fluorescence quenching, although there were minor changes in the capacity for quenching and in its induction kinetics. High-light-induced violaxanthin deepoxidation to zeaxanthin was not affected, although the pool size of these pigments was decreased slightly. We conclude that CP29 and CP26 are unlikely to be sites for nonphotochemical quenching.

References

Feb 15, 1992·European Journal of Biochemistry·R Bassi, P Dainese
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·N A DolganovA R Grossman
Feb 8, 1994·Biochimica Et Biophysica Acta·S Jansson
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·R G WaltersP Horton
Aug 12, 2000·Journal of Experimental Botany·K Maxwell, G N Johnson

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Citations

Feb 22, 2011·Journal of Bioenergetics and Biomembranes·Lorenzo FerroniSimonetta Pancaldi
Sep 7, 2005·Photosynthesis Research·Jun Minagawa, Yuichiro Takahashi
Sep 20, 2005·Photosynthesis Research·Ryouichi Tanaka, Ayumi Tanaka
Jul 3, 2010·Photosynthesis Research·Jonathan A D Neilson, Dion G Durnford
Jun 25, 2013·Photosynthesis Research·Julia ZaksGraham R Fleming
Aug 17, 2011·The Journal of Biological Chemistry·Yuliya MiloslavinaAlfred R Holzwarth
Dec 12, 2007·The Journal of Biological Chemistry·Giulia BonenteRoberto Bassi
Mar 25, 2009·The Journal of Biological Chemistry·Nico BetterleRoberto Bassi
Dec 30, 2008·Plant & Cell Physiology·José G García-CerdánWolfgang P Schröder
Nov 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Peter Horton
Jan 20, 2004·Plant Physiology·Ulrika GanetegStefan Jansson
Aug 13, 2002·The Plant Cell·Dafna ElradArthur R Grossman
Sep 25, 2003·Annual Review of Plant Biology·Gunnar Oquist, Norman P A Huner
Jan 30, 2014·Annual Review of Plant Biology·Jean-David Rochaix
Jan 9, 2009·The Journal of Biological Chemistry·Matteo BallottariRoberto Bassi
Mar 21, 2006·FEBS Letters·Sophie CrouchmanPeter Horton
Mar 16, 2011·Journal of Photochemistry and Photobiology. B, Biology·Roberta Croce, Herbert van Amerongen

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