PMID: 8584762Oct 20, 1995Paper

Antisense oligodeoxynucleotide to a delta-opioid receptor blocks the antinociception induced by cold water swimming

Regulatory Peptides
H MizoguchiL F Tseng

Abstract

The type of opioid receptors in the spinal cord involved in antinociception induced by cold water swimming (CWS) was studied in male ICR mice. Mice were submitted to CWS for 3 min and antinociception was measured 7 min after the CWS by the tail-flick test. Intrathecal (i.t.) injection of naltrindole (NTI, 5 micrograms) which blocked i.t. DPDPE ([D-Pen2,D-Pen5]en-kephalin, 5 micrograms)-induced antinociception, blocked the CWS-induced antinociception. On the other hand, i.t. injection of CTOP (D-Phe-Cys-Try-D-Try-Orn-Thr-Pen-Thr-NH2, 50 ng) and norbinaltorphimine (nor-BNI, 5 micrograms) which blocked i.t. DAMGO ([D-Ala2,NHPhe4,Gly-ol]enkephalin, 10 ng)- and U50,488H (trans(+/-)-3,4-dichloro-N-methyl-N-[2-(1-pyrrolidinyl)- cyclohexyl]benzeneacetamide, 75 micrograms)-induced antinociception, respectively, did not block CWS-induced antinociception. Intrathecal pretreatment with antisense oligodeoxynucleotide to delta-opioid receptor mRNA (delta-AS oligo, 163 pmol) once a day for 1 to 3 days caused a time-dependent attenuation of CWS-induced antinociception. delta-AS at doses from 1.6 to 163 pmol pretreated i.t. for 3 days caused a dose-dependent blockade of CWS-induced antinociception. However, i.t. pretreatment with mismatch oligo...Continue Reading

References

Dec 28, 1992·Science·C J EvansR H Edwards
Dec 14, 1984·Science·G W TermanJ C Liebeskind
Oct 17, 1980·European Journal of Pharmacology·J L Hylden, G L Wilcox
Feb 1, 1994·Trends in Pharmacological Sciences·C Wahlestedt

❮ Previous
Next ❯

Citations

Nov 16, 2005·Behavioural Pharmacology·Soheila Fazli-TabaeiMohammad-Reza Zarrindast
Aug 18, 2006·Behavioural Pharmacology·Soheila Fazli-TabaeiMohammad-Reza Zarrindast
Jan 24, 2002·The Journal of Pharmacology and Experimental Therapeutics·Hsiang-En WuLeon F Tseng
Oct 15, 2003·The Journal of Pharmacology and Experimental Therapeutics·Hsiang-En WuLeon F Tseng
Mar 5, 2004·The Journal of Pharmacology and Experimental Therapeutics·Hsiang-En WuLeon F Tseng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.