Abstract
To explore whether local blockade of T-box expressed in T cells (T-bet) expression in the lungs could lead to airway inflammation. Twenty-four rats were randomly divided into 4 groups: saline group, ovalbumin (OVA)-sensitized group, nonsense group, and the antisense group. The OVA-sensitized rats were sensitized and challenged with OVA, and the rats in the nonsense and antisense groups were subjected to an aerosol delivery of the nonsense and antisense oligonucleotides (AS-ODN) of T-bet (0.1%, w/v). The levels of interferon-gamma (IFN-gamma), interleukin-4 (IL-4), and IL-5 in the bronchoalveolar lavage fluid (BALF) were detected by ELISA, and the mRNA and the protein expression of T-bet and GATA-3 genes were examined by in situ hybridization and Western blot analysis, respectively. The expression of T-bet mRNA and protein in the lungs of the rats in the antisense group were inhibited effectively. The lungs of the rats in the antisense and OVA-sensitized groups showed eosinophil and lymphocyte inflammatory infiltration, and eosinophilia located predominantly around the airways. The number of GATA-3 mRNA-positive cells and the level of GATA-3 protein in the lungs of the rats in the antisense and the OVA-sensitized groups signific...Continue Reading
References
May 16, 1997·Cell·W Zheng, R A Flavell
Aug 22, 1997·The Journal of Biological Chemistry·D H ZhangA Ray
May 4, 1999·International Archives of Allergy and Immunology·O M Kon, A B Kay
Oct 19, 1999·The Journal of Clinical Investigation·A Ray, L Cohn
Dec 10, 1999·Nature·S T Holgate
Apr 13, 2000·Cell·S J SzaboL H Glimcher
May 12, 2000·Biochimica Et Biophysica Acta·S T Crooke
Jun 8, 2001·The Journal of Experimental Medicine·S FinottoM F Neurath
Jul 18, 2001·The Journal of Experimental Medicine·Y ZhangH Huang
Oct 2, 2001·The Journal of Biological Chemistry·G T SchwengerC J Sanderson
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·A A LighvaniJ J O'Shea
Jan 12, 2002·Science·Susetta FinottoLaurie H Glimcher
Apr 26, 2002·Current Opinion in Immunology·Jane L Grogan, Richard M Locksley
Dec 26, 2002·Annual Review of Immunology·Susanne J SzaboLaurie H Glimcher
Apr 27, 2004·Journal of Pharmacological Sciences·Eunjung KoHyunsu Bae
Sep 24, 2004·Journal of Theoretical Biology·Andrew YatesJaroslav Stark
Oct 23, 2004·Clinical and Experimental Immunology·S A RitzM Jordana
Nov 13, 2004·Immunity·Amy E Lovett-RackeMichael K Racke
Jan 8, 2005·BMJ : British Medical Journal·Jonathan Kimmelman
Jan 22, 2005·Science·Eun Sook HwangLaurie H Glimcher
Feb 19, 2005·Science·Jennifer Couzin, Jocelyn Kaiser
Mar 17, 2005·Planta medica·Jun-Li YuGui-Fang Cheng
May 20, 2005·American Journal of Respiratory and Critical Care Medicine·Henning WeigtArmin Braun
Aug 24, 2005·Acta Pharmacologica Sinica·Zu-qun WuHua-hao Shen
Sep 24, 2005·American Journal of Respiratory and Critical Care Medicine·Benjamin A RabyScott T Weiss
Citations
Mar 17, 2007·Chinese Journal of Integrative Medicine·Gang WangTing-qian Li
Jul 29, 2011·Cytokine·Mei GaoJie Sun
Jun 16, 2015·Chinese Medicine·Hong Ping ZhangGang Wang
Aug 8, 2015·Journal of Cellular Physiology·Alessandra MiceraStefano Bonini
Mar 19, 2016·Respirology : Official Journal of the Asian Pacific Society of Respirology·Hong Ping ZhangGang Wang
Sep 7, 2018·Experimental and Therapeutic Medicine·Shanshan QiHao Chen