May 12, 2000

Antisense properties of peptide nucleic acid

Biochimica Et Biophysica Acta
H J LarsenPeter E Nielsen

Abstract

Peptide nucleic acid (PNA) is a nucleic acid mimic in which the deoxyribose phosphate backbone has been replaced by a pseudo-peptide polymer to which the nucleobases are linked. PNA-oligomers can be synthesized in relatively large amounts, are highly stable in biological environments, and bind complementary DNA and RNA targets with remarkably high affinity and specificity. Thus PNA possesses many of the properties desired for a good antisense agent. Until recently, limited uptake of PNA into cells has been the major obstacle for applying PNA as an antisense agent in cell cultures and in vivo. Here, the antisense properties of PNA in vitro and in vivo will be reviewed. In particular, we will focus on recent observations indicating that PNA equipped with or without various uptake moieties may function as an efficient and gene-specific inhibitor of translation in Escherichia coli and in certain mammalian cell types.

Mentioned in this Paper

Peptide Nucleic Acids
Drug Carriers
Alkalescens-Dispar Group
Neurons
Pseudo brand of pseudoephedrine
Uptake
Cell Culture Techniques
Neurotensin type 1 receptor
Phosphate Measurement
Protein Biosynthesis

About this Paper

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