PMID: 11925034Apr 2, 2002Paper

Antisense repression of cytosolic phosphoglucomutase in potato (Solanum tuberosum) results in severe growth retardation, reduction in tuber number and altered carbon metabolism

Planta
Alisdair R FernieR N Trethewey

Abstract

The aim of this work was to investigate the role of cytosolic phosphoglucomutase (PGM; EC 5.4.2.2) in the regulation of carbohydrate metabolism. Many in vitro studies have indicated that PGM plays a central role in carbohydrate metabolism; however, until now the importance of this enzyme in plants has not been subject to reverse-genetics investigations. With this intention we cloned the cytosolic isoform of potato PGM (StcPGM) and expressed this in the antisense orientation under the control of the CaMV 35 S promoter in potato plants. We confirmed that these plants contained reduced total PGM activity and that loss in activity was due specifically to a reduction in cytosolic PGM activity. These plants were characterised by a severe phenotype: stunted aerial growth combined with limited root growth and a reduced tuber yield. Analysis of the metabolism of these lines revealed that leaves of these plants were inhibited in sucrose synthesis whereas the tubers exhibited decreased levels of sucrose and starch as well as decreased levels of glycolytic intermediates but possessed unaltered levels of adenylates. Furthermore, a broader metabolite screen utilising GC-MS profiling revealed that these lines contained altered levels of sever...Continue Reading

Citations

Sep 27, 2003·Plant Physiology·Ute Roessner-TunaliAlisdair R Fernie
Oct 21, 2010·Plant Physiology·Barbara EgliSebastian Streb
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rhonda C MeyerThomas Altmann
Dec 28, 2010·Journal of Plant Physiology·Joost T van DongenAlisdair R Fernie
Apr 17, 2007·Current Opinion in Plant Biology·Anna LytovchenkoAlisdair R Fernie
May 12, 2004·Current Opinion in Plant Biology·Alisdair R FernieLee J Sweetlove
May 28, 2014·Biological Reviews of the Cambridge Philosophical Society·Douglas S Glazier
Jul 6, 2013·Biotechnology Advances·Abdellatif BahajiJavier Pozueta-Romero
Oct 27, 2004·Journal of Plant Physiology·Josef Berüter
Mar 23, 2010·Current Opinion in Plant Biology·Christina Kühn, Christopher P L Grof
Jan 18, 2005·Current Opinion in Plant Biology·Susan I Gibson
Mar 25, 2018·Physiologia Plantarum·Amir Mahboubi, Totte Niittylä
Jun 14, 2005·Plant & Cell Physiology·Francisco José MuñozJavier Pozueta-Romero
Jan 18, 2020·The Plant Journal : for Cell and Molecular Biology·Toshihiro ObataAlisdair R Fernie
May 2, 2003·Planta·Chun Yao LiEliezer E Goldschmidt
Sep 2, 2009·Plant Signaling & Behavior·Joost J B Keurentjes, Ronan Sulpice
Nov 30, 2012·Plant Biotechnology Journal·Uwe Sonnewald, Jens Kossmann
Apr 1, 2009·Plant Science : an International Journal of Experimental Plant Biology·Piero Morandini

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