PMID: 11607409Jul 1, 1993Paper

Antisense repression of the chloroplast triose phosphate translocator affects carbon partitioning in transgenic potato plants

Proceedings of the National Academy of Sciences of the United States of America
J W RiesmeierW B Frommer

Abstract

The major chloroplast envelope membrane protein E29 is central for the communication between chloroplasts and cytosol. It has been identified as the triose phosphate translocator (TPT) exporting the primary products of the Calvin cycle (i.e., triose phosphates and 3-phosphoglycerate) out of the chloroplast in a strict counter exchange for Pi. To study the in vivo role of the TPT, transgenic potato plants were constructed that have a reduced expression of the TPT at both the RNA and protein level due to antisense inhibition. Chloroplasts isolated from these plants show a 20-30% reduction with respect to their ability to import Pi. The reduced TPT activity leads to a reduction of maximal photosynthesis by 40-60%, to a change in carbon partitioning into starch at the expense of sucrose and amino acids, and to an increase of the leaf starch content by a factor of approximately 3. At early developmental stages the inhibited plants are retarded in growth compared to the wild type.

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Citations

Apr 9, 2005·Trends in Plant Science·H Ekkehard NeuhausUwe Sonnewald
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Edurne Baroja-FernándezJavier Pozueta-Romero
Apr 10, 2004·Journal of Experimental Botany·M G PoolmanD A Fell
Mar 30, 2004·Journal of Experimental Botany·Andreas P M WeberLars M Voll
Aug 1, 1991·European Journal of Biochemistry·J JoyardR Douce
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Ulf-Ingo Flugge
May 3, 2005·Annual Review of Plant Biology·Andreas P M WeberUlf-Ingo Flügge
Jan 1, 2002·The Arabidopsis Book·Uwe Ludewig, Wolf B Frommer
Nov 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gianni TeoAbhaya M Dandekar
Jun 25, 2014·Antioxidants & Redox Signaling·Peter Geigenberger, Alisdair R Fernie
Dec 30, 2009·Molecular Plant·Nicole Linka, Andreas P M Weber
Aug 12, 2000·Journal of Experimental Botany·M G PoolmanS Thomas
Aug 12, 2000·Journal of Experimental Botany·F KauderD Heineke
Jan 9, 1999·The Plant Journal : for Cell and Molecular Biology·J KehrJ Fisahn
Jun 14, 2005·Plant & Cell Physiology·Francisco José MuñozJavier Pozueta-Romero
Dec 8, 2006·The Biochemical Journal·Samuel C ZeemanAlison M Smith
Mar 15, 2019·Journal of Experimental Botany·Alan M McClain, Thomas D Sharkey
Sep 27, 2003·Plant Physiology·Ute Roessner-TunaliAlisdair R Fernie
Aug 1, 2004·The New Phytologist·Samuel C ZeemanAlison M Smith
Dec 10, 1998·Plant Physiology·J SchleucherT D Sharkey

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