Antisense RNA regulation by stable complex formation in the Enterococcus faecalis plasmid pAD1 par addiction system

Journal of Bacteriology
Keith E WeaverSmita Patel

Abstract

The par stability determinant, encoded by the Enterococcus faecalis plasmid pAD1, is the only antisense RNA regulated postsegregational killing system identified in gram-positive bacteria. Because of the unique organization of the par locus, the par antisense RNA, RNA II, binds to its target, RNA I, at relatively small, interspersed regions of complementarity. The results of this study suggest that, rather than targeting the antisense bound message for rapid degradation, as occurs in most other antisense RNA regulated systems, RNA I and RNA II form a relatively stable, presumably translationally inactive complex. The stability of the RNA I-RNA II complex would allow RNA I to persist in an untranslated state unless or until the encoding plasmid was lost. After plasmid loss, RNA II would be removed from the complex, allowing translational activation of RNA I. The mechanism of RNA I activation in vivo is unknown, but in vitro dissociation experiments suggest that active removal of RNA II, for example by a cellular RNase, may be required.

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G M DunnyD B Clewell
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H C Wong, S Chang
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K GerdesS Molin
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J TomizawaT Som
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P StougaardK Nordström
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Y IkeD B Clewell
Feb 1, 1995·Molecular Microbiology·N Delihas
Aug 1, 1995·Molecular Microbiology·L S WaldbeserJ H Crosa
Sep 1, 1996·Molecular Microbiology·T Franch, K Gerdes
Aug 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·R A Lease, M Belfort
Apr 5, 2002·Advances in Genetics·E Gerhart H WagnerPascale Romby
May 22, 2002·Biochimica Et Biophysica Acta·Sabine Brantl
Mar 26, 2003·Nucleic Acids Research·Ruth HershbergHanah Margalit
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Ciarán Condon
Sep 17, 2003·Genes & Development·Eric MasséSusan Gottesman

❮ Previous
Next ❯

Citations

Aug 24, 2013·Nucleic Acids Research·Natalie Jahn, Sabine Brantl
Aug 6, 2014·Toxins·Jia Wen, Elizabeth M Fozo
Mar 23, 2007·Current Opinion in Microbiology·Keith E Weaver
Mar 23, 2007·Current Opinion in Microbiology·Kenn Gerdes, E Gerhart H Wagner
Jan 25, 2011·Microbial Biotechnology·Jens KrollAlexander Steinbüchel
Jan 11, 2012·Molecular Microbiology·Natalie JahnSabine Brantl
Mar 27, 2015·FEMS Microbiology Reviews·Sabine Brantl, Natalie Jahn
Jan 14, 2017·PloS One·Chen Chris Gong, Stefan Klumpp
Nov 14, 2015·The Journal of Biological Chemistry·Christin MeißnerSabine Brantl
Jan 10, 2017·Frontiers in Microbiology·Yoonji KimJihwan Hwang
Mar 9, 2019·Microbiology Spectrum·Keith E Weaver

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.