Antitermination by GlpP, catabolite repression via CcpA and inducer exclusion triggered by P-GlpK dephosphorylation control Bacillus subtilis glpFK expression

Molecular Microbiology
E DarbonJosef Deutscher

Abstract

The Bacillus subtilis glpFK operon encoding the glycerol transport facilitator (GlpF) and glycerol kinase (GlpK) is induced by glycerol-3-P and repressed by rapidly metabolizable sugars. Carbon catabolite repression (CCR) of glpFK is partly mediated via a catabolite response element cre preceding glpFK. This operator site is recognized by the catabolite control protein A (CcpA) in complex with one of its co-repressors, P-Ser-HPr or P-Ser-Crh. HPr is a component of the phosphoenolpyruvate:sugar phosphotransferase system (PTS), and Crh is an HPr homologue. The hprK-encoded HPr kinase phosphorylates HPr and Crh at Ser-46. But in neither ccpA nor hprK mutants was expression of a glpF'-lacZ fusion relieved from CCR, as a second, CcpA-independent CCR mechanism implying the terminator tglpFK, whose formation is prevented by the glycerol-3-P-activated antiterminator GlpP, is operative. Deletion of tglpFK led to elevated expression of the glpF'-lacZ fusion and to partial relief from CCR. CCR completely disappeared in DeltatglpFK mutants carrying a disruption of ccpA or hprK. The tglpFK-requiring CCR mechanism seems to be based on insufficient synthesis of glycerol-3-P, as CCR of glpFK was absent in ccpA mutants growing on glycerol-3-P o...Continue Reading

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Citations

Mar 8, 2005·Archives of Microbiology·Wade C Winkler
Mar 9, 2004·Journal of Molecular Biology·Grégory BoëlJosef Deutscher
Sep 2, 2008·Applied and Environmental Microbiology·Doris RittmannVolker F Wendisch
Nov 14, 2006·Applied and Environmental Microbiology·Alain MazéDouwe van Sinderen
May 27, 2008·Journal of Bacteriology·Biju JosephWerner Goebel
Dec 8, 2009·Journal of Bacteriology·Alain BizziniAxel Hartke
Nov 4, 2005·Journal of Bacteriology·Graciela L LorcaMilton H Saier
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Sep 24, 2004·Journal of Molecular Microbiology and Biotechnology·María de Fátima AlvarezFernando Sesma
Jan 18, 2006·Journal of Molecular Microbiology and Biotechnology·Rana HerroJosef Deutscher
Dec 22, 2006·Journal of Molecular Microbiology and Biotechnology·Vicente MonederoJosef Deutscher
Aug 19, 2007·Journal of Molecular Microbiology and Biotechnology·Sophie BeaufilsAxel Hartke
Jun 28, 2011·BMC Microbiology·Anette McLeodLars Axelsson
Feb 10, 2009·Bioscience, Biotechnology, and Biochemistry·Yasutaro Fujita
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Oct 8, 2014·Journal of Basic Microbiology·Zhiwei NiBin Liu
Feb 3, 2006·FEMS Microbiology Letters·Nicolas SauvageotAxel Hartke
Jun 15, 2007·Critical Reviews in Microbiology·Zijun Xiao, Ping Xu
May 11, 2004·Microbiology·Safia ArousYann Héchard

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