Antithrombin prevents reperfusion-induced hepatic apoptosis by enhancing insulin-like growth factor-I production in mice

Critical Care Medicine
Naoaki HaradaNaomi Nakagata

Abstract

Antithrombin (AT) reduces ischemia/reperfusion-induced liver injury by increasing release of calcitonin gene-related peptide (CGRP) from sensory neurons. Because CGRP increases the production of insulin-like growth factor-I (IGF-I), an antiapoptotic factor, it is possible that AT prevents apoptosis by increasing IGF-I production. We examined this possibility in the present study. Prospective, randomized, controlled study. University laboratory. Male C57BL/6 wild-type mice and alphaCGRP-deficient mice weighing 16-23 g. AT (250 units/kg) was intravenously administered to mice subjected to hepatic ischemia/reperfusion. Liver injury was evaluated by determining changes in serum levels of alanine aminotransferase after ischemia/reperfusion. Hepatic apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining. AT reduced ischemia/reperfusion-induced liver injury and enhanced increases in hepatic tissue levels of IGF-I in wild-type mice, although it did not reduce liver injury or enhance increases in hepatic tissue levels of IGF-I in alphaCGRP-deficient mice. Reperfusion-induced hepatic apoptosis was markedly suppressed by AT in wild-type mice, but not in alphaCGRP-deficient mice. Pre...Continue Reading

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Citations

Jul 15, 2009·Translational Research : the Journal of Laboratory and Clinical Medicine·Naoaki HaradaKenji Okajima
Jun 16, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naoaki HaradaKenji Okajima
Jul 21, 2011·Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·Kenji OkajimaNaoaki Harada

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Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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