Antithrombotic and fibrinolytic system of human endothelial cells seeded on PTFE: the effects of surface modification of PTFE by ammonia plasma treatment and ECM protein coatings

Biomaterials
A Lu, R Sipehia

Abstract

The aim of this study was to determine the effects of ECM protein coatings and surface modification of PTFE on the ability of seeded human endothelial cells (EC) to secrete prostacyclin (PGI2), plasminogen inhibitor-1 (PAI-1) and tissue plasminogen activator (t-PA). PTFE surfaces were modified by a novel surface modification technique based on ammonia plasma. Fibronectin, collagen type-1 and gelatin-coated ammonia plasma modified PTFE and unmodified PTFE surfaces were employed and compared in this study. All ammonia plasma modified surfaces showed similar secretions of PGI2 compared to non-modified PTFE surfaces. With the exception of gelatin-coated modified PTFE, seeded EC seeded on all modified PTFE showed lower levels of PAI-1 secretion compared to those seeded on unmodified PTFE. The specific activity of t-PA secreted by EC seeded on ammonia plasma modified and fibronectin coated modified PTFE showed increases of 100 and 30%, respectively, when compared to their unmodified counterparts. Our studies show that EC seeded on modified PTFE have ability to secrete PGI2 that modulates the early phase of thrombus formation. Furthermore, superior t-PA profile, along with lower levels of PAl-1 suggest that ammonia plasma modification...Continue Reading

References

Jun 1, 1987·Journal of Biomedical Materials Research·P B van WachemW G van Aken
Mar 13, 1987·Science·C T Esmon
Feb 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G M Rodgers
Nov 1, 1987·Journal of Biomedical Materials Research·P B van WachemW G van Aken
Sep 1, 1985·Journal of Biomedical Materials Research·K L Bentley, R J Klebe
May 1, 1982·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S Moncada
Sep 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·P N RaghunathE S Barnathan
Jun 1, 1995·The Journal of Thoracic and Cardiovascular Surgery·J C ZhangB R Binder
Jan 1, 1994·Journal of Biomaterials Science. Polymer Edition·H J GriesserJ G Steele
Jan 1, 1993·Biomaterials, Artificial Cells, and Immobilization Biotechnology : Official Journal of the International Society for Artificial Cells and Immobilization Biotechnology·R Sipehia
Nov 1, 1993·Annals of Surgery·M G Davies, P O Hagen
Jan 1, 1993·Biomaterials, Artificial Cells, and Immobilization Biotechnology : Official Journal of the International Society for Artificial Cells and Immobilization Biotechnology·R SipehiaC Wu
Feb 1, 1996·Journal of Biomedical Materials Research·K Kottke-MarchantR E Marchant
Nov 24, 1999·Biochimica Et Biophysica Acta·C F ChuR Sipehia
Jul 1, 1959·The Journal of Pathology and Bacteriology·A S TODD

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Citations

Jun 21, 2005·Journal of Materials Science. Materials in Medicine·Qinggang TanJia-Cong Shen
Jan 12, 2011·Journal of Materials Science. Materials in Medicine·Yun XiaSubbu S Venkatraman
Sep 1, 2005·Journal of Biomaterials Science. Polymer Edition·Cheng LiMir Imran
Feb 28, 2006·Tissue Engineering·Philippe FernandezLaurence Bordenave
Feb 24, 2007·Biomaterials·Alison P McGuigan, Michael V Sefton
Sep 16, 2004·Journal of Biomedical Materials Research. Part a·Cheng LiMir Imran
Oct 1, 2004·Hemodialysis International·Cheng LiFermin Tio
May 21, 2008·Journal of Biomedical Materials Research. Part a·Wei HeSeeram Ramakrishna
Nov 18, 2006·Journal of Biomedical Materials Research. Part a·Mingyu SheMichael V Sefton
Jan 5, 2019·Annals of Biomedical Engineering·Allison PostElizabeth Cosgriff-Hernandez
Nov 30, 2018·Annals of Biomedical Engineering·Allison PostElizabeth Cosgriff-Hernandez
Aug 22, 2020·ACS Applied Materials & Interfaces·Peter Matthew Paul T FowlerYung Chang

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