Antitumor activities of Quercetin and Green Tea in xenografts of human leukemia HL60 cells

Scientific Reports
Andrana Karla CalgarottoSara Teresinha Olalla Saad

Abstract

Quercetin is one of the most abundant flavonoids, present in fruits and vegetables and has been shown to have multiple properties capable of reducing cell growth in cancer cells. Green tea is a widely consumed beverage, known for a potential source of free radical scavenging and anti-cancer activities. Herein, we investigate the in vivo antitumor efficacy of quercetin and green tea in human leukemia. Human tumors were xenografted into NOD/SCID mice. Quercetin and green tea reduced tumor growth in HL-60 xenografts accompanied by decreased expression of anti-apoptotic proteins, BCL-2, BCL-XL and MCL-1 and increased expression of BAX, a pro-apoptotic protein. Moreover, caspase-3 was activated to a greater extent after quercetin and green tea treatment. Quercetin and green tea also mediated G1 phase cell cycle arrest in HL-60 xenografts. Treatment with quercetin and green tea induced conversion of LC3-I to LC3-II as well as activation of autophagy proteins, suggesting that quercetin and green tea initiate the autophagic progression. We have provided evidence that quercetin and green tea induces signaling at the level of apoptosis, cell cycle and autophagy which converge to antigrowth effects in HL-60 xenograft mice suggesting that ...Continue Reading

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Citations

Jun 1, 2018·International Journal of Molecular Sciences·Maiara de Souza OliveiraDaniel Pereira Bezerra
Dec 28, 2018·Journal of Cellular Physiology·Adel NaimiSaeed Solali
Oct 1, 2019·Archives of Physiology and Biochemistry·Milad AshrafizadehSaeed Samarghandian
Jul 12, 2020·International Journal of Molecular Sciences·Beatriz Castejón-VegaJosé M Alvarez-Suarez
Dec 11, 2019·International Journal of Molecular Sciences·Guo-Yi TangHua-Bin Li
Mar 17, 2020·Cell & Bioscience·Asma VafadarHamed Mirzaei
Sep 16, 2020·International Journal of Molecular Sciences·Monica BenvenutoRoberto Bei
Mar 7, 2021·Pharmaceuticals·Paula Garcia-OliveiraMiguel Angel Prieto
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Saleh A AlmatroodiArshad Husain Rahmani
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Coralia CotoraciAnca Hermenean
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhicheng ZhangXin Zhang
Nov 18, 2018·Journal of Ethnopharmacology·Ana Carolina B da C RodriguesDaniel P Bezerra

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Methods Mentioned

BETA
xenograft
xenografts
Protein Assay

Software Mentioned

Prism
GraphPad

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