Antitumor activity of Brazilian red propolis fractions against Hep-2 cancer cell line

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Caroline Olivieri da Silva FrozzaJ A P Henriques

Abstract

Continuous increases in the rates of tumor diseases have highlighted the need for identification of novel and inexpensive antitumor agents from natural sources. In this study, we investigated the effects of enriched fraction from hydroalcoholic Brazilian red propolis extract against Hep-2 cancer cell line. Initially 201 fractions were arranged in 12 groups according to their chromatographic characteristics (A-L). After an in vitro cell viability screening, J and L were further selected as promising enriched fractions for this study. The chemical characterization was performed and Biochanin A, Formononetin, and Liquiritigenin compounds were quantified. Through MTT viability assay and morphological changes observed by Giemsa and DAPI staining, the results showed that red propolis inhibited cancer cells growth. Flow cytometry results indicated effects that were partly mediated through programmed cell death as confirmed by externalization of phosphatidylserine, DNA cleaved assay, increase at SUB G1-G0 phase in cell cycle analysis and loss of mitochondrial membrane potential. In conclusion, our results demonstrated that red propolis enriched fractions promoted apoptotic effects in human cancer cells through the mechanisms involving ...Continue Reading

Citations

Apr 25, 2019·Biofouling·Stela Lima Farias MirandaBruno Bueno-Silva
May 10, 2020·Biomolecules·Felipe Mendes de Andrade de CarvalhoRicardo Luiz Cavalcanti de Albuquerque-Júnior
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Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lingchen TaoFuliang Hu
Jun 26, 2021·Frontiers in Microbiology·Cíntia M Dos SantosVeridiana V de Rosso
Jul 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Marcelo Augusto Duarte SilveiraUNKNOWN BeeCovid Team
Jun 12, 2020·Journal of Natural Products·Thais P BanzatoRoberto G S Berlinck

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