Antitumor activity of eosinophils activated by IL-5 and eotaxin against hepatocellular carcinoma

DNA and Cell Biology
Sayo KataokaAkira Tominaga

Abstract

We examined the antitumor effects of eosinophils to explore the potential of eosinophils as effector cells in tumor cytotoxicity. We expressed eotaxin in hepatocellular carcinoma cells, MH134, and injected them into either normal or IL-5 TG mice intradermally and monitored cell growth. In normal mice, growth of MH134 cells containing the expression plasmid pCXN2-eotaxin was similar to that of vector-transfected MH134 cells for a period of 2 weeks, suggesting that expression of eotaxin does not change the growth rate of tumor cells. In IL-5 TG mice, however, the growth of eotaxin expressing MH134 cells was significantly suppressed. LPS induced eosinophils to produce TNF-alpha to kill MH134 cells in vitro. Intratumor injection of LPS is effective to kill MH134-pCXN2 and MH134-pCXN2-eotaxin only in normal mice. Administration of anti-CD4 or anti-CD8 antibodies suppressed growth of MH134-pCXN2-eotaxin cells compared with control antibodies, suggesting that T cells may interfere with immunity against MH134. Administration of anti-IL-5Ralpha and anti-asialo GM1 antibodies enhanced growth of MH134-pCXN2-eotaxin cells, suggesting involvement of eosinophils and NK cells in suppression of tumor cell growth. Although we cannot exclude the...Continue Reading

References

Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·M E RothenbergP Leder
Feb 17, 1997·The Journal of Experimental Medicine·M E RothenbergP Leder
Mar 1, 1997·The Journal of Clinical Investigation·A W MouldP S Foster
May 29, 1998·The New England Journal of Medicine·M E Rothenberg
Apr 5, 2000·The Journal of Clinical Investigation·H Z ShiP F Weller
Jun 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R SalcedoJ J Oppenheim
Mar 27, 2002·Nature Reviews. Immunology·Bernhard HomeyAlbert Zlotnik
Feb 5, 2003·The Journal of Experimental Medicine·Joerg MattesChristopher Parish

❮ Previous
Next ❯

Citations

Nov 21, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Takuya WatanabeMinoru Nomoto
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yong TangLouis M Weiner
Jan 9, 2018·Oncoimmunology·Gilda VarricchiGiovanna Schiavoni
Dec 25, 2015·The Journal of Biological Chemistry·Inga KretschmerJens W Fischer
Feb 26, 2008·Expert Opinion on Biological Therapy·Karin JöhrerRichard Greil
Feb 16, 2018·EMBO Molecular Medicine·Eline HaspeslaghBart N Lambrecht
Jun 6, 2020·Frontiers in Immunology·Alireza Labani-MotlaghAngelica Loskog
Jun 20, 2020·Cell Communication and Signaling : CCS·Sarah B GittoDeborah A Altomare
Jul 18, 2020·Nature Reviews. Cancer·Sharon Grisaru-TalAriel Munitz
Mar 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ljubov SimsonChristopher R Parish
Apr 15, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hideto NishikadoHajime Karasuyama
Mar 27, 2019·Cellular & Molecular Immunology·Guillaume HabifEmilie Narni-Mancinelli
Sep 16, 2020·Archives of Gynecology and Obstetrics·Theresa VilsmaierUNKNOWN SUCCESS Study Group
Nov 28, 2018·Cancers·Daisuke NishikawaNobuhiro Hanai
Apr 30, 2014·Cancer Immunology Research·Benjamin P Davis, Marc E Rothenberg
Jan 23, 2019·Cancer Immunology Research·Hadar ReichmanAriel Munitz
Jan 12, 2021·Frontiers in Cell and Developmental Biology·Yang LiuZewen Song
Nov 26, 2020·Cancers·Enrico MaggiFrancesco Annunziato
Jul 13, 2021·Signal Transduction and Targeted Therapy·Huakan ZhaoYongsheng Li
May 11, 2010·Journal of Theoretical Biology·Raluca EftimieDavid J D Earn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.