Antitumor activity of gefitinib in malignant rhabdoid tumor cells in vitro and in vivo

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Yasumichi KuwaharaTohru Sugimoto

Abstract

Malignant rhabdoid tumor (MRT) is a rare and highly aggressive neoplasm of young children. Current treatments have had only limited success. Epidermal growth factor receptor (EGFR) was found recently to be expressed on MRT cell lines. Gefitinib (trade name Iressa) is an oral and selective EGFR-tyrosine kinase inhibitor and has been demonstrated to be effective in inhibiting the proliferation of cancer cells in vivo as well as in clinical trials. This encouraged us to examine the antitumor effects of gefitinib on MRT cells in vitro and in vivo. The expression of EGFR in two MRT tumors and two MRT cell lines (MP-MRT-AN and KP-MRT-NS), established from these two tumor tissues, was examined by immunohistochemistry, immunofluorescence, and immunoblot. The effect of gefitinib on EGFR phosphorylation was examined by immunoblot. The effects of gefitinib on cell growth and apoptosis were examined by cell growth assay and terminal deoxynucleotidyl transferase-mediated nick end labeling assay. The in vivo effect of gefitinib was assessed in athymic mice that had been xenografted with MRT cells. The expression of EGFR was detected in both tumor tissues and cell lines. Gefitinib inhibited EGFR-phosphorylation (IC(50) < 0.1 micromol/L) and i...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Jan 1, 1982·Journal of Cellular Biochemistry·M D WaterfieldB Ozanne
Jan 12, 1996·The Journal of Biological Chemistry·Y R ChenT H Tan
Oct 21, 1996·International Journal of Cancer. Journal International Du Cancer·H IshidaT Sawada
Jul 27, 1999·International Journal of Cancer. Journal International Du Cancer·T SugimotoT Sawada
May 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Malcolm RansonEric K Rowinsky
Oct 17, 2002·Drugs·Christine R Culy, Diana Faulds
Mar 22, 2003·Experimental Cell Research·Thomas HolbroNancy E Hynes
May 16, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Masahiro FukuokaJosé Baselga

❮ Previous
Next ❯

Citations

Jul 22, 2008·Cancer Chemotherapy and Pharmacology·Kristen FosterCynthia Wright
Aug 30, 2008·Cancer Chemotherapy and Pharmacology·Efstathia GiannopoulouHaralabos P Kalofonos
Aug 30, 2011·Biochemical and Biophysical Research Communications·Yoshiki KatsumiHajime Hosoi
May 8, 2007·Biochemical and Biophysical Research Communications·Shinichi TamuraTohru Sugimoto
Sep 30, 2008·International Journal of Cancer. Journal International Du Cancer·Motonobu WatanabeTatsutoshi Nakahata
Oct 19, 2007·Pediatric Blood & Cancer·Valerie LaroucheEric Bouffet
Dec 1, 2012·International Journal of Cancer. Journal International Du Cancer·Yasumichi KuwaharaBernard E Weissman
Nov 8, 2016·Expert Review of Anticancer Therapy·Paul H Huang
May 18, 2007·The Biochemical Journal·Alexandre ArcaroBarbara Fischer
May 5, 2020·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Colin KennyMaureen J O'Sullivan
Feb 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yoshiki KatsumiTohru Sugimoto
Feb 2, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Helena MontanuyJordi Surrallés

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anorexia Nervosa

Anorexia nervosa (AN) is a psychiatric condition characterized by severe weight loss and secondary problems associated with malnutrition. Here is the latest research on AN.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Related Papers

Biochemical and Biophysical Research Communications
Shinichi TamuraTohru Sugimoto
International Journal of Cancer. Journal International Du Cancer
Marie P PiechockiFulvio Lonardo
Proceedings of the National Academy of Sciences of the United States of America
Mary TsikitisGanjam V Kalpana
© 2021 Meta ULC. All rights reserved