Antitumor and HIV-1 Reverse Transcriptase Inhibitory Activities of a Hemagglutinin and a Protease Inhibitor from Mini-Black Soybean.

Evidence-based Complementary and Alternative Medicine : ECAM
Xiu Juan Ye, Tzi Bun Ng

Abstract

Protease inhibitors (PIs) and hemagglutinins are defense proteins produced by many organisms. From Chinese mini-black soybeans, a 17.5-kDa PI was isolated using chromatography on Q-Sepharose, SP-Sepharose, and DEAE-cellulose. A 25-kDa hemagglutinin was purified similarly, but using Superdex 75 instead of DEAE-cellulose in the final step. The PI inhibited trypsin and chymotrypsin (IC(50) = 7.2 and 8.8 μM). Its trypsin inhibitory activity was stable from pH 2 to pH 13 and from 0°C to 70°C. The hemagglutinin activity of the hemagglutinin was stable from pH 2 to pH 13 and from 0°C to 75°C. The results indicated that both PI and hemagglutinin were relatively thermostable and pH-stable. The trypsin inhibitory activity was inhibited by dithiothreitol, signifying the importance of the disulfide bond to the activity. The hemagglutinating activity was inhibited most potently by D (+)-raffinose and N-acetyl-D-galactosamine, suggesting that the hemagglutinin was specific for these two sugars. Both PI and hemagglutinin inhibited HIV-1 reverse transcriptase (IC(50) = 3.2 and 5.5 μM), proliferation of breast cancer cells (IC(50) = 9.7 and 3.5 μM), and hepatoma cells (IC(50) = 35 and 6.2 μM), with relatively high potencies.

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Citations

Jan 12, 2013·Applied Biochemistry and Biotechnology·Yau Sang ChanTzi Bun Ng
Dec 5, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Shanmugam Hemaiswarya, Mukesh Doble
Mar 12, 2015·Proteomics·Mehran Dastmalchi, Sangeeta Dhaubhadel
Jul 19, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Yau Sang ChanTzi Bun Ng
May 5, 2017·Nutrients·Kumar Ganesan, Baojun Xu
Apr 26, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Ajay Kumar GautamSameer S Bhagyawant

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Methods Mentioned

BETA
ion exchange chromatography
electrophoresis
ELISA
gel filtration
ion

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