Antitumor effect of a novel adeno-associated virus vector targeting to telomerase activity in tumor cells

Acta Biochimica Et Biophysica Sinica
Yigang WangXinyuan Liu

Abstract

Telomerase activity is a wide tumor marker. Human telomerase reverse transcriptase (hTERT), the catalytic subunit of the telomerase, is transcriptionally upregulated exclusively in about 90% of cancer cells. In this study, we constructed a novel adeno-associated virus (AAV) vector containing the human interferon-beta (hIFN-beta) gene under the control of hTERT promoter (AAV-hTERT-hIFN-beta) and investigated its antitumor effect against various human cancer cells in vitro. AAV-hTERT-hIFN-beta displayed cancer-specific hIFN-beta expression and cytotoxicity. The cytotoxic ratio was positively correlated with the time length of infection. AAV-hTERT-hIFN-beta-mediated apoptotic morphology was observed by transmission electron microscopy. Flow cytometry assay also revealed that the cytotoxicity of AAV-hTERT-hIFN-beta was mainly an apoptotic process. These data indicate that AAV in combination with hTERT-mediated therapeutic gene expression may open new possibilities for long-lasting and targeting gene therapy of varieties of cancers.

Citations

Jan 13, 2016·World Journal of Gastroenterology : WJG·Yi-Gang WangYan-Fang Sun
Jan 1, 2011·Cancers·Lyse A NorianThomas S Griffith
Feb 3, 2021·Cancer Treatment and Research Communications·Ali Salimi-JedaAsghar Abdoli

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