Antitumor Effect of GO-PEG-DOX Complex on EMT-6 Mouse Breast Cancer Cells

Cancer Biotherapy & Radiopharmaceuticals
Jinyin YanNing Li

Abstract

Doxorubicin (DOX) can be used to treat malignant tumors, but with multiple adverse effects. Graphene oxide-polyethylene glycol (GO-PEG) is a novel nanoscale carrier material and can elevate solubility and biocompatibility of drugs. This study prepared a GO-PEG-DOX complex, whose toxicity and antitumor effects were evaluated on mouse EMT-6 breast cancer cells. GO-PEG-DOX complex was prepared for calculating the drug carrier rate of DOX on GO-PEG by MV approach. EMT-6 cells were treated with 40 μg/mL GO-PEG, 1 μg/mL DOX, or 40 μg/mL +1 μg/mL GO-PEG-DOX for 72 h of incubation. Cells without treatment were considered the control group. Cell survival rate and apoptotic rate were tested at different time points. GO-PEG and GO-PEG-DOX complex were successfully prepared with satisfactory solubility. After 72 h of incubation, EMT-6 cells after GO-PEG-DOX treatment had significantly higher survival rate than GO-PEG group (p < 0.05). All three treatment groups had significantly elevated apoptotic rates than control group (p < 0.05). GO-PEG-DOX group had much more apoptosis (p < 0.05 compared with DOX group). Moreover, with elongated treatment time, all groups showed decreased survival rate (p < 0.05). GO-PEG did not reduce the cytotoxicit...Continue Reading

References

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Citations

Feb 3, 2019·Journal of Cellular and Molecular Medicine·Yanfen ZouLizhou Sun
May 9, 2021·International Journal of Pharmaceutics·JinLing ZhengHongFei Chen
Jul 17, 2021·Journal of Nanobiotechnology·Guangman CuiGuangyu Yao

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Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
acetylation
surgical resection

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