PMID: 8439988Jan 1, 1993Paper

Antitumor effects of a novel monoclonal antibody with high binding affinity to ganglioside GD3

Cancer Immunology, Immunotherapy : CII
S OhtaN Hanai

Abstract

Ganglioside GD3, which is one of the major gangliosides expressed on the cell surface human tumors of neuroectodermal origin, has been studied as a target molecule for passive immunotherapy. We established ten kinds of anti-GD3 monoclonal antibodies (mAb) of the mouse IgG3 subclass by immunization with purified GD3 and melanoma cells. One of the established mAb, KM641, showed major reactivity with GD3 and minor reactivity with GQ1b out of 11 common gangliosides in an enzyme-linked immunosorbent assay. Immunostaining of gangliosides, separated on thin-layer chromatography plates, using KM641 revealed that most of the melanoma cell lines contained immunoreactive GD3 and GD3-lactone at a high level, but only the adrenal gland and the urinary bladder out of 21 human normal tissues had immunoreactive GD3. In immunofluorescence, KM641 bound to a variety of living tumor cell lines especially melanoma cells, including some cell lines to which another anti-GD3 mAb R24, established previously, failed to bind. High-affinity binding of KM641 to a tumor cell line was quantified by Scatchard analysis (Kd = 1.9 x 10(-8) M). KM641 exerted tumor-killing activity in the presence of effector cells or complement against melanoma cells expressing G...Continue Reading

References

Feb 15, 1979·European Journal of Biochemistry·J PortoukalianJ F Doré
Jun 19, 1992·International Journal of Cancer. Journal International Du Cancer·H BernhardW Dippold
Dec 1, 1991·Journal of Biochemistry·S WataraiT Yasuda
Jan 1, 1991·Cancer Immunology, Immunotherapy : CII·K MujooM G Rosenblum
Jan 24, 1991·Nature·G Winter, C Milstein
Jun 15, 1991·Cancer·M H RavindranathR F Irie
Jan 1, 1989·Acta Neuropathologica·X HeD D Bigner
Mar 15, 1989·Journal of the National Cancer Institute·D IliopoulosD Herlyn
Oct 1, 1988·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S Vadhan-RajA N Houghton
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R F Irie, D L Morton
Jan 1, 1987·Journal of the National Cancer Institute·T TsuchidaR F Irie
Sep 1, 1987·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·N K CheungN A Berger
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I HellströmK E Hellström
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A N HoughtonL J Old
Oct 5, 1972·Biochimica Et Biophysica Acta·M HolmL Svennerholm
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D A ChereshR A Reisfeld
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W G DippoldL J Old
Mar 15, 1982·International Journal of Cancer. Journal International Du Cancer·M Y YehK E Hellström
Sep 1, 1963·Journal of Neurochemistry·L SVENNERHOLM

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Citations

Jun 1, 1993·Cancer Immunology, Immunotherapy : CII·K ShitaraN Hanai
Jan 3, 1994·Journal of Immunological Methods·K ShitaraN Hanai
Oct 6, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·T SuzawaM Yamasaki
Jan 30, 2007·Cancer Biotherapy & Radiopharmaceuticals·Pierre L TriozziAndres Forero
Jan 30, 2007·Cancer Biotherapy & Radiopharmaceuticals·Andres ForeroRobert M Conry
Aug 17, 2012·Future Oncology·Catherine RabuLindy Durrant
Sep 4, 1996·International Journal of Cancer. Journal International Du Cancer·H FukumotoN Saijo
Jul 15, 1999·International Journal of Cancer. Journal International Du Cancer·I BergmanN K Cheung
Oct 2, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A M ScottL J Old
Sep 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nidia Alvarez-RuedaStéphane Birklé
May 1, 2021·International Journal of Molecular Sciences·Tetsuya Okuda

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