Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells.

Journal of Zhejiang University. Science. B
Guo-qing DingYue Zhang

Abstract

Our objective was to construct a recombinant bacillus Calmette-Guérin vaccine (rBCG) that secretes human interferon-alpha 2b (IFNα-2b) and to study its immunogenicity and in vitro antitumor activity against human bladder cancer cell lines T24 and T5637. The signal sequence BCG Ag85B and the gene IFNα-2b were amplified from the genome of BCG and human peripheral blood, respectively, by polymerase chain reaction (PCR). The two genes were cloned in Escherichia coli-BCG shuttle-vector pMV261 to obtain a new recombinant plasmid pMV261-Ag85B-IFNα-2b. BCG was transformed with the recombinant plasmid by electroporation and designated rBCG-IFNα-2b. Mononuclear cells were isolated from human peripheral blood (PBMCs) and stimulated with rBCG-IFNα-2b or wild type BCG for 3 d, and then cultured with human bladder cancer cell lines T24 and T5637. Their cytotoxicities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. BCG was successfully transformed with the recombinant plasmid pMV261-Ag85B-IFNα-2b by electroporation and the recombinant BCG (rBCG-IFNα-2b) was capable of synthesizing and secreting cytokine IFNα-2b. PBMC proliferation was enhanced significantly by rBCG-IFNα-2b, and the cytotoxicity of PB...Continue Reading

References

Jan 1, 1992·European Urology·T L Ratliff
May 23, 1998·The Journal of Urology·A S BelldegrunR D Williams
Aug 5, 2000·Current Opinion in Urology·W Hassen, M J Droller
May 9, 2001·The Journal of Urology·C DurekA Böhle
Feb 11, 2004·Journal of Immunotherapy·Judith ArnoldSven Brandau
Aug 18, 2004·The Journal of Urology·Michael A O'DonnellUNKNOWN National Bacillus Calmette-Guerin/Interferon Phase 2 Investigator Group
Sep 17, 2004·The Journal of Urology·Henrik SuttmannSven Brandau
Feb 17, 2007·International Journal of Urology : Official Journal of the Japanese Urological Association·Yasuyo ShintaniToshiaki Shinka
Dec 2, 2008·Journal of Experimental & Clinical Cancer Research : CR·Daher C ChadePriscila M Andrade
Jul 20, 2010·Immunotherapy·Anton B AlexandroffAndrew M Jackson

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Citations

Oct 12, 2012·Applied Microbiology and Biotechnology·Karine Rech BegniniFabiana Kömmling Seixas
Mar 22, 2015·Applied Microbiology and Biotechnology·K R BegniniF K Seixas
Jun 2, 2016·Therapeutic Advances in Urology·Akhil MuthigiPiyush K Agarwal
Sep 19, 2014·Expert Review of Anticancer Therapy·Yonghua WangXinsheng Wang

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