Antitumor efficacy improved by local delivery of species-specific endostatin

Journal of Neurosurgery
Peter C HuszthyPer Oyvind Enger

Abstract

Conflicting results have been reported concerning the antitumor efficacy of the angiogenesis inhibitor endostatin. This may be due to differences in the biological distribution of endostatin between studies or to the varying biological efficacies of the different protein forms that were examined. To address this issue, the authors used a local delivery approach in which each tumor cell secreted endostatin, providing uniform endostatin levels throughout the tumors. This allowed a direct assessment of the biological efficacy of soluble endostatin in vivo. The authors genetically engineered BT4C gliosarcoma cells so that they would stably express and secrete either the human or murine form of endostatin. Endostatin-producing cells or mock-infected cells were implanted intracerebrally in syngeneic BD-IX rats. The antitumor efficacy of endostatin was evaluated on the basis of survival data and tumor volume comparisons. In addition, microvascular parameters were assessed. The authors confirmed the continuous release of endostatin by the BT4C cells. A magnetic resonance imaging-assisted comparison of tumor volumes revealed that local production of murine endostatin significantly inhibited tumor growth. Notably, 40% of the animals in t...Continue Reading

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Citations

Dec 4, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Kai WangXiaoyuan Chen
Mar 10, 2007·European Journal of Pharmacology·Ye-Fen XuGen-Xing Xu
Oct 12, 2010·Journal of Angiogenesis Research·Ariel Gilert, Marcelle Machluf
Mar 4, 2010·BMC Biotechnology·Danielle B RodriguesLigia Morganti

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BETA
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fluorescence microscopy
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LUCIA G
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