PMID: 11345206May 10, 2001Paper

Antitumor immunity induced by irradiated tumor cells producing macrophage colony-stimulating factor

International Journal of Hematology
S SuzuK Motoyoshi

Abstract

We previously reported that administration into mice of mouse lymphoid leukemia L1210 cells engineered to secrete macrophage colony-stimulating factor (M-CSF) could lead to tumor rejection. Here, we demonstrate that inoculation with irradiated M-CSF-producing cells protects mice against a subsequent challenge with unmodified parental tumor cells. We used 2 experimental protocols: the inoculation with irradiated M-CSF-producing L1210 cells (EM5) before the challenge with parental cells and after the challenge with parental cells. Both protocols effectively improved the survival rate of mice compared with protocols in which irradiated non-M-CSF-producing L1210 cells (EM-mock) were inoculated. Inoculation with 1 x 10(2) irradiated EM5 cells was sufficient to prolong the survival time of mice subsequently challenged with 1 x 10(4) parental cells. In vivo depletion experiments with administration of antibodies suggested the involvement of CD4+ T cells, CD8+ T cells, and natural killer (NK) cells in the antitumor effect. Consistent with these findings, the cytotoxic T lymphocyte activity of splenocytes from EM5-inoculated mice was higher than that from EM-mock-inoculated mice, and L1210 tumors were heavily infiltrated by CD4+ T cells...Continue Reading

References

Sep 11, 1990·Nucleic Acids Research·S Mizushima, S Nagata
Apr 1, 1995·European Journal of Immunology·G Schmidt-Wolf, I G Schmidt-Wolf
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Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·K OshikawaN S Yang
May 3, 2000·The Journal of Clinical Investigation·S Agha-Mohammadi, M T Lotze

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Citations

May 12, 2017·Journal of Virology·Dhong Hyun Lee, Homayon Ghiasi

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