Antitumoral Potential of Lansbermin-I, a Novel Disintegrin from Porthidium lansbergii lansbergii Venom on Breast Cancer Cells

Current Topics in Medicinal Chemistry
Leonel Montealegre-SanchezEliécer Jiménez-Charris

Abstract

Disintegrins from snake venoms bind with high specificity cell surface integrins, which are important pharmacological targets associated with cancer development and progression. In this study, we isolated a disintegrin from the Porthidium lansbergii lansbergii venom and evaluated its antitumoral effects on breast cancer cells. The isolation of the disintegrin was performed on RP-HPLC and the inhibition of platelet aggregation was evaluated on human platelet-rich plasma. The inhibition of cell adhesion was also evaluated in vitro on cultures of cell lines by the MTT method as well as the inhibition of breast cancer cell migration by the wound healing assay. The binding of the disintegrin to integrin subunits was verified by flow cytometry and confocal microscopy. Finally, inhibition of angiogenesis was assessed in vitro on HUVEC cells and the concentration of VEGF was measured in the cellular supernatants. The disintegrin, named Lansbermin-I, is a low molecular weight protein (< 10 kDa) that includes an RGD on its sequence identified previously. Lansbermin-I showed potent inhibition of ADP and collagen-induced platelet aggregation on human plasma and also displayed inhibitory effects on the adhesion and migration of breast cance...Continue Reading

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Citations

Nov 16, 2019·Current Topics in Medicinal Chemistry·Juliana Pavan Zuliani, Andreimar Martins Soares
Mar 12, 2020·Current Topics in Medicinal Chemistry·Hua Guo, Quan-Ping Diao
May 20, 2020·Seminars in Cancer Biology·Félix A Urra, Ramiro Araya-Maturana
Jul 15, 2020·Biochemical Pharmacology·Axel H SchönthalFrancis S Markland
Mar 18, 2021·European Journal of Pharmacology·Bushra AkhtarMuhammad Irfan Anwar
Aug 11, 2020·Toxicon: X·Olamide Tosin OlaobaDulce Helena Ferreira de Souza

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