Antitumoural hydroxyapatite nanoparticles-mediated hepatoma-targeted trans-arterial embolization gene therapy: in vitro and in vivo studies

Liver International : Official Journal of the International Association for the Study of the Liver
Gaopeng LiBaoming Luo

Abstract

Absence of curative treatment creates urgent need for new strategies for unresectable hepatoma. Based on former discoveries of good liver cell compatibility, safety and tumour-specific inhibition of hydroxyapatite nanoparticles (nHAP), this work tries to make nHAP serve as gene vector in the hepatoma-targeted trans-arterial embolization (TAE) gene therapy to elevate and synergize the therapeutic efficacy of TAE and target gene therapy. Following dosage and ratio optimization, polypolex formed by surface modified nHAP and p53 expressing plasmid was applied in vitro for human hepatoma HePG2 cell, and then in vivo for rabbit hepatic VX2 tumour by injection of polypolex/lipodoil emulsion to the hepatic artery in a tumour-target manner. In vitro, the polypolex transfected only about 5% HepG2 cells, but can elevate the inhibition of its growth and apoptosis in a much more degree while keeping safe to the normal hepatocyte line, L02. In vivo, the emulsion, with better dispersion than the polypolex and more specific tumour-target than lipiodol, mediated specific 4% p53 expression and antitumoural nanoparticle retention in the target tumour site, also significantly reduced tumour growth and prolonged the animal survival times more than ...Continue Reading

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Citations

Oct 15, 2013·International Journal of Nanomedicine·Shengli DongGaopeng Li
Jan 15, 2016·Colloids and Surfaces. B, Biointerfaces·Wang YanhuaShengmin Zhang
Jun 2, 2020·Drug Development and Industrial Pharmacy·Behrad GhiasiVuk Uskoković
Dec 21, 2017·Chemical Society Reviews·Chao QiPeng Huang
Dec 15, 2016·ACS Applied Materials & Interfaces·Rammohan DevulapallyRamasamy Paulmurugan

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