Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine

The Journal of Venomous Animals and Toxins Including Tropical Diseases
Ricardo Teixeira-AraújoCarlos Corrêa-Netto

Abstract

Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom against South American rattlesnake (Crotalus durissus terrificus) bites is produced by public institutions based on the guidelines defined by the regulatory agency of the Brazilian Ministry of Health, ANVISA. However, each institution uses its own mixture of rattlesnake venom antigens. Previous works have shown that crotamine, a toxin found in Crolatus durissus venom, shows marked individual and populational variation. In addition, serum produced from crotamine-negative venoms fails to recognize this molecule. In this work, we used an antivenomics approach to assess the cross-reactivity of crotalic antivenom manufactured by IVB towards crotamine-negative venom and a mixture of crotamine-negative/crotamine-positive venoms. We show that the venom mixture containing 20% crotamine and 57% crotoxin produced a strong immunogenic response in horses. Antivenom raised against this venom mixture reacted with most venom components including crotamine and crotoxin, i...Continue Reading

References

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Citations

May 5, 2020·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Lídia J TasimaAnita M Tanaka-Azevedo
Dec 12, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Isadora Sousa de OliveiraEliane Candiani Arantes
Sep 9, 2019·Toxicon : Official Journal of the International Society on Toxinology·Letícia Lopes-de-SouzaCarlos Chávez-Olórtegui
May 29, 2020·International Journal of Biological Macromolecules·Patrícia D Vaz de MeloCarlos Chavez-Olortegui
Aug 24, 2020·Toxicon : Official Journal of the International Society on Toxinology·Jia-Qi LiXiang Ji
Mar 7, 2018·Toxicon : Official Journal of the International Society on Toxinology·José María Gutiérrez
Apr 25, 2021·Toxicon : Official Journal of the International Society on Toxinology·Roberto Ponce-LópezAlejandro Olvera-Rodríguez
Mar 19, 2019·Toxicon : Official Journal of the International Society on Toxinology·R A BaumD E Keyler
Sep 28, 2021·Toxicon : Official Journal of the International Society on Toxinology·Esteban Ortiz-PradoJuan S Izquierdo-Condoy

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Methods Mentioned

BETA
ELISA

Software Mentioned

ANVISA

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