PMID: 8970989Dec 1, 1996Paper

Antiviral activity of influenza virus M1 zinc finger peptides

Journal of Virology
E H NasserD J Bucher

Abstract

Matrix protein (M1) of influenza virus inhibits its own polymerase; this suggested that a peptide segment of M1 with inhibitory properties could serve as an antiviral agent. A peptide synthesized to the Zn2+ finger region of the M1 sequence of influenza virus strain A/PR/8/34 centered around amino acids residues 148 to 166 was shown earlier to be 1,000-fold more effective as a polymerase inhibitor than M1. This peptide, designated peptide 6, represents a Zn2+ finger which includes a 7-residue "loop" and a 4-residue "tail" in addition to the 4 residues on either side of the loop involved in coordination of Zn2+. We have now demonstrated antiviral activity for this peptide in microassays measuring inhibition of the viral cytopathic effect. When the peptide was introduced into tissue culture 5 min after viral challenge with A/PR/8/34, antiviral activity was seen at levels as low as 0.1 nM; on a molar basis, the peptide was shown to be 1,000- to 2,500-fold more effective than ribavirin or amantadine. Antiviral activity was seen with addition of the peptide up to 1 h after viral infection; however, little or no activity was seen at later times, suggesting that viral replication is inhibited at an early stage, possibly at the level o...Continue Reading

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Citations

Nov 19, 2011·Antiviral Chemistry & Chemotherapy·Hongbing JiangTetsuya Toyoda
Nov 20, 2002·Journal of Virology·Teresa Liu, Zhiping Ye
Sep 10, 1999·Antiviral Chemistry & Chemotherapy·J M ColacinoW G Laver
Jun 27, 2013·Medicinal Research Reviews·Evelien Vanderlinden, Lieve Naesens
Oct 23, 2015·Viruses·Sylvie SkalickovaRene Kizek
Feb 1, 2005·FEMS Immunology and Medical Microbiology·Umesh C Chaturvedi, Richa Shrivastava
Jun 8, 2010·Current Microbiology·Yongjin WangXiaoming Wang
Aug 10, 1999·Journal of Virology·Z YeR A Levandowski
Sep 1, 1997·Virology·O P Zhirnov, H D Klenk
Mar 1, 1997·Antimicrobial Agents and Chemotherapy·A K JuddR W Sidwell

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