Abstract
The bark, leaves, and flowers of Paulownia trees have been used in traditional Chinese medicine to treat infectious and inflammatory diseases. We investigated the antiviral effects of Paulownia tomentosa flowers, an herbal medicine used in some provinces of P. R. China for the treatment of skin rashes and blisters. Dried flowers of P. tomentosa were extracted with methanol and tested for antiviral activity against enterovirus 71 (EV71) and coxsackievirus A16 (CAV16), the predominant etiologic agents of hand, foot, and mouth disease in P. R. China. The extract inhibited EV71 infection, although no effect was detected against CAV16 infection. Bioactivity-guided fractionation was performed to identify apigenin as an active component of the flowers. The EC50 value for apigenin to block EV71 infection was 11.0 µM, with a selectivity index of approximately 9.3. Although it is a common dietary flavonoid, only apigenin, and not similar compounds like naringenin and quercetin, were active against EV71 infection. As an RNA virus, the genome of EV71 has an internal ribosome entry site that interacts with heterogeneous nuclear ribonucleoproteins (hnRNPs) and regulates viral translation. Cross-linking followed by immunoprecipitation and rev...Continue Reading
References
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Dec 1, 1994·Archives of Pharmacal Research·K H KangM K Park
Nov 5, 1999·Phytotherapy Research : PTR·K H KangC K Lee
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Somashe NiranjanakumariMariano A Garcia-Blanco
Apr 29, 2004·Planta medica·Ting-Fu JiangYan-Ping Shi
Feb 19, 2008·The Journal of Infectious Diseases·Zhao-Hong LiShun-Hua Chen
Sep 18, 2008·The Journal of General Virology·Jing-Yi LinShin-Ru Shih
Apr 3, 2009·Journal of Virology·Jing-Yi LinMei-Ling Li
Mar 23, 2010·Pharmaceutical Research·Sanjeev Shukla, Sanjay Gupta
May 13, 2010·Virology Journal·Yan ZhangWenbo Xu
Oct 22, 2010·The Lancet Infectious Diseases·Tom SolomonMong How Ooi
Feb 18, 2011·The Pediatric Infectious Disease Journal·Edmond MaThomas Tsang
Sep 29, 2011·Antiviral Research·Xiaoqing ChenErguang Li
Apr 3, 2012·Journal of Virological Methods·Ying-Ju LinCheng-Wen Lin
Mar 5, 2013·Phytochemistry·Alice NavrátilováKarel Šmejkal
Mar 5, 2013·Journal of Ethnopharmacology·Jingjing WangGuang Yang
Apr 5, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaoqing ChenYu Jin
Apr 30, 2013·Bioorganic & Medicinal Chemistry·Jung Keun ChoKi Hun Park
May 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Daniel ArangoAndrea I Doseff
Jun 4, 2013·RNA Biology·Jeffrey D LevengoodBlanton S Tolbert
Citations
Feb 3, 2016·Chinese Medicine·Mengjie WangHongxi Xu
Apr 17, 2016·Carbohydrate Polymers·Chao ZhaoZheng Xing
Apr 11, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Guilherme Rabelo CoelhoGiuseppina Negri
Aug 2, 2018·Virology Journal·Chenguang YaoYanhong Wei
Aug 24, 2017·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Y ZhangY Yang
Feb 6, 2016·Oncotarget·Mengjie WangMing-Liang He
Apr 12, 2019·Bioscience Reports·Haifeng YangChunmao Jiang
Aug 19, 2020·Animal Biotechnology·Mahmoud AlagawanyMohamed E Abd E-Hack
Jul 4, 2015·Microbiology and Immunology·Zhi-Hui LiLizeng Qin
Jul 12, 2017·PeerJ·Zhe WangYabing Cao
Nov 19, 2019·BioMed Research International·Minqian WangKit Yam
Apr 22, 2017·Scientific Reports·Sin-Guang ChenHung-Yao Ho
Aug 28, 2020·Frontiers in Pharmacology·Bachir Benarba, Atanasio Pandiella