Antiviral activity of phenanthrenes from the medicinal plant Bletilla striata against influenza A virus

BMC Complementary and Alternative Medicine
Ya ShiDongqing Cheng

Abstract

Influenza represents a serious public health concern. The emergence of resistance to anti-influenza drugs underlines the need to develop new drugs. This study aimed to evaluate the anti-influenza viral activity and possible mechanisms of 12 phenanthrenes from the medicinal plant Bletilla striata (Orchidaceae family). Twelve phenanthrenes were isolated and identified from B. striata. Influenza virus A/Sydney/5/97 (H3N2) propagated in embryonated chicken eggs was used. Phenanthrenes mixed with the virus were incubated at 37 °C for 1 h and then inoculated into 9-day-old embryonated chicken eggs via the allantoic route to survey the antiviral activity in vivo. A (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS)-based assay was performed to evaluate the reduction of cytopathic effect induced by H3N2 on Madin-Darby canine kidney (MDCK) cells. The hemagglutination inhibition assay was used to study the blockage of virus receptors by the phenanthrenes, and the neuraminidase (NA) inhibition assay to evaluate the effects of the release of virus. The synthesis of influenza viral matrix protein mRNA in response to compound treatment was measured by real-time polymerase chain reaction. This stu...Continue Reading

References

Oct 1, 1991·Journal of Chemotherapy·R AquinoM L Stein
Dec 24, 2005·The New England Journal of Medicine·Menno D de JongJeremy Farrar
Feb 3, 2007·Current Medicinal Chemistry·Jianzhi GongJie Zhang
Feb 5, 2008·Phytochemistry·Adriána KovácsJudit Hohmann
Apr 18, 2008·Nature·Andrew RambautEdward C Holmes
Aug 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xianghong JingRobert A Lamb
Sep 15, 2009·Current Medicinal Chemistry·Jianzhi GongWenfang Xu
Jul 24, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Christine Korteweg, Jiang Gu
Nov 10, 2010·Journal of Agricultural and Food Chemistry·Kailiang WangQingmin Wang
May 20, 2011·BMC Infectious Diseases·Kristian ThorlundLehana Thabane
Nov 15, 2013·The Journal of General Virology·Thomas O EdingerSilke Stertz
Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·Deepak PatilAbhay Chowdhary
Mar 4, 2014·PLoS Genetics·Matthieu FollJeffrey D Jensen
May 29, 2014·Current Opinion in Virology·Aeron C Hurt
Feb 28, 2015·Nature Reviews. Drug Discovery·Florian Krammer, Peter Palese
Mar 12, 2015·Journal of Natural Products·Chao-Dong QianZhi-Shan Ding
Nov 26, 2015·Vaccine·Leslie A ReperantAlbert D M E Osterhaus

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Citations

Mar 7, 2020·The American Journal of Chinese Medicine·Yu-Pei ChenYu-Chiang Hung
Apr 5, 2018·Archives of Microbiology·Ana Rita de Toledo-PizaCristina Adelaide Figueiredo
Jun 5, 2018·BioMed Research International·Meiya LiFusheng Jiang
Oct 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Syam MohanAsaad Khalid

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Datasets Mentioned

BETA
BS-2012-I

Methods Mentioned

BETA
PCR
Fluorescence

Software Mentioned

GraphPad
GraphPad Prism
SPSS

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