Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo

Virology Journal
Han HuQigai He

Abstract

Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation. In this study, we evaluated the activities of five broad-spectrum antimicrobial peptides (AMPs) against several important swine-origin pathogenic viruses by TCID50 assay. Plaque reduction assay and cell apoptosis assay were also used to test the activity of the peptides. Protection effect of piscidin against pseudorabies virus (PRV) was also examined in mouse model. Piscidin (piscidin 1), caerin (caerin 1.1) and maculatin (maculatin 1.1) could inhibit PRV by direct interaction with the virus particles in a dose-dependent manner and they could also protect the cells from PRV-induced apoptosis. Among the peptides tested, piscidin showed the strongest activity against PRV. Moreover, in vivo assay showed that piscidin can reduce the mortality of mice infected with PRV. In vitro and in vivo experiments indicate that piscidin has antiviral activity against PRV.

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Citations

May 20, 2020·International Journal of Peptide Research and Therapeutics·Garima Agarwal, Reema Gabrani
Nov 18, 2020·Antibiotics·Guihong PenJianhua Wang
Apr 21, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Hannah CetukSergei Sukharev
May 3, 2021·Microbial Pathogenesis·Subhash Singh SolankiSyed Azmal Ali
Oct 15, 2021·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Yiyi ZhouDaojun Hong

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Methods Mentioned

BETA
ELISA

Software Mentioned

SPSS

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