Antiviral effects of β-defensin derived from orange-spotted grouper (Epinephelus coioides)

Fish & Shellfish Immunology
Minglan GuoQiwei Qin

Abstract

Defensins are a group of small antimicrobial peptides playing an important role in innate host defense. In this study, a β-defensin cloned from liver of orange-spotted grouper, Epinephelus coioides, EcDefensin, showed a key role in inhibiting the infection and replication of two kinds of newly emerging marine fish viruses, an enveloped DNA virus of Singapore grouper iridovirus (SGIV), and a non-enveloped RNA virus of viral nervous necrosis virus (VNNV). The expression profiles of EcDefensin were significantly (P < 0.001) up-regulated after challenging with Lipopolysaccharide (LPS), SGIV and Polyriboinosinic Polyribocytidylic Acid (polyI:C) in vivo. Immunofluorescence staining observed its intracellular innate immune response to viral infection of SGIV and VNNV. EcDefensin was found to possess dual antiviral activity, inhibiting the infection and replication of SGIV and VNNV and inducting a type I interferon-related response in vitro. Synthetic peptide of EcDefensin (Ec-defensin) incubated with virus or cells before infection reduced the viral infectivity. Ec-defensin drastically decreased SGIV and VNNV titers, viral gene expression and structural protein accumulation. Grouper spleen cells over-expressing EcDefensin (GS/pcDNA-Ec...Continue Reading

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Citations

Mar 7, 2014·Pharmaceuticals·Jorge A Masso-Silva, Gill Diamond
Feb 25, 2014·Applied Microbiology and Biotechnology·Randy Chi Fai CheungTzi Bun Ng
Jul 24, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tao LiangGui-Wei Zou
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Nov 19, 2018·Developmental and Comparative Immunology·Yongcan ZhouChunsheng Liu
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Jul 16, 2021·Frontiers in Veterinary Science·Yewande O FasinaSierra Muzquiz

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