Antiviral effects of grape seed extract against feline calicivirus, murine norovirus, and hepatitis A virus in model food systems and under gastric conditions

Food Microbiology
Snehal S JoshiDoris H D'Souza

Abstract

Grape seed extract (GSE) has antiviral activities against hepatitis A virus (HAV) and human norovirus surrogates (feline calicivirus (FCV-F9) and murine norovirus (MNV-1)). The objectives of this study were to determine (1) time and dose-dependence of GSE against FCV-F9, MNV-1, and HAV at room temperature (RT) and 37 °C over 24 h; and (2) GSE effects in model foods (apple juice (AJ) and 2% milk) and simulated gastric conditions at 37 °C. Viruses at ∼5 log PFU/ml were treated with 0.5-8 mg/ml GSE prepared in water, AJ, milk or gastric juices, or water over 24 h at RT or 37 °C. Infectivity of triplicate treatments was evaluated using plaque assays. GSE effects increased with time and concentration. GSE at 1 mg/ml in AJ reduced MNV-1 to undetectable levels after 1 h and by 1 log in milk after 24 h. GSE at 1 and 2 mg/ml in AJ reduced HAV to undetectable levels after 1 h, while 2 and 4 mg/ml GSE in milk caused ∼1 log reduction after 24 h. GSE at 2 mg/ml in intestinal fluid reduced FCV-F9, MNV-1 and HAV to undetectable levels after 6 h. GSE appears to be a suitable natural option for foodborne viral reduction.

References

Jul 5, 2003·Biological & Pharmaceutical Bulletin·Kayoko TakagiJun-ichi Sawada
Apr 20, 2005·The Journal of Infectious Diseases·Anna T PalamaraEnrico Garaci
Jul 2, 2005·Molecular Nutrition & Food Research·Kathrin KahleElke Richling
Oct 26, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Manjinder KaurChapla Agarwal
Jan 14, 2009·Phytotherapy Research : PTR·Marjan Nassiri-Asl, Hossein Hosseinzadeh
Apr 7, 2010·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Irina C ChisNicoleta Decea
Apr 14, 2010·International Journal of Molecular Sciences·En-Qin XiaHua-Bin Li
Jun 4, 2010·The Journal of Toxicological Sciences·Aamar Al-HabibMohammad Afzal
Jul 23, 2011·Foodborne Pathogens and Disease·Xiaowei SuDoris H D'Souza
Mar 19, 2013·Food Microbiology·Xiaowei Su, Doris H D'Souza
May 17, 2014·International Journal of Biological Macromolecules·Paulraj Kanmani, Jong-Whan Rhim

❮ Previous
Next ❯

Citations

Jun 15, 2016·International Journal of Food Microbiology·Hajime TakahashiBon Kimura
May 30, 2020·Journal of Applied Microbiology·Walter Randazzo, Gloria Sánchez
Apr 27, 2018·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Michiko TakahashiBon Kimura
Feb 6, 2019·Food and Environmental Virology·Lucas Ariel Totaro GarciaMarkus Nagl
Jan 10, 2017·Frontiers in Pharmacology·Wei-Chun ChenJin-Ching Lee
Jun 24, 2020·Frontiers in Medicine·Giuseppe AnnunziataEttore Novellino
Jul 28, 2020·Foodborne Pathogens and Disease·Hyojin KimMi Sook Chung
Sep 15, 2017·BMC Complementary and Alternative Medicine·Sabrina M NoritakeMendel Friedman
Dec 31, 2018·International Journal of Food Microbiology·Irene FalcóGloria Sánchez
May 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Walter RandazzoGloria Sánchez
Apr 21, 2021·Food Microbiology·Snehal S Joshi, Doris H D'Souza
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jolanta SarowskaIrena Choroszy-Król
Aug 7, 2021·Comprehensive Reviews in Food Science and Food Safety·Andreza Salles BaroneAna Elizabeth Cavalcante Fai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.