Antiviral effects of hepatitis B virus S gene-specific anti-gene locked nucleic acid in transgenic mice

World Journal of Clinical Cases
Shu-Rong XiaoYi-bin Deng

Abstract

To assess the antiviral effects of hepatitis B virus (HBV) S gene-specific anti-gene locked nucleic acid (LNA) in transgenic mice. Thirty HBV transgenic mice were acclimatized to laboratory conditions and positive for serum HBV surface antigen (HBsAg) and HBV DNA, were randomly divided into 5 groups (n = 7), including negative control (blank control, unrelated sequence control), positive control (lamivudine, anti-sense-LNA), and anti-gene-LNA experimental group. LNA was injected into transgenic mice by tail vein while lamivudine was administered by gavage. Serum HBV DNA and HBsAg levels were determined by fluorescence-based PCR and enzyme-linked immune sorbent assay, respectively. HBV S gene expression amounts were assessed by reverse transcription polymerase chain reaction. Positive rates of HBsAg in liver cells were evaluated immunohistochemistry. Average rate reductions of HBsAg after treatment on the 3rd, 5th, and 7th days were 32.34%, 45.96%, and 59.15%, respectively. The inhibitory effect of anti-gene-LNA on serum HBsAg peaked on day 7, with statistically significant differences compared with pre-treatment (0.96 ± 0.18 vs 2.35 ± 0.33, P < 0.05) and control values (P < 0.05 for all). Average reduction rates of HBV DNA on t...Continue Reading

References

Jul 1, 1997·Pharmaceutical Research·R I MahatoE Tomlinson
Apr 25, 2002·Nucleic Acids Research·Jens KurreckVolker A Erdmann
Aug 17, 2005·Current Medicinal Chemistry. Anti-cancer Agents·Faye A RogersPeter M Glazer
Mar 28, 2008·Nature·Joacim ElménSakari Kauppinen
May 8, 2008·Biochimie·Philippe SimonCarine Giovannangeli
Apr 20, 2011·Biochimie·Anirban Mukherjee, Karen M Vasquez
Jan 11, 2012·Clinical Microbiology Reviews·Jasmine SamalPerumal Vivekanandan
Apr 25, 2013·World Journal of Gastroenterology : WJG·Xue-Cheng SunXiao-Hua Ye
Feb 22, 2014·Methods in Molecular Biology·Faisal Reza, Peter M Glazer
May 31, 2014·World Journal of Gastroenterology : WJG·Ceen-Ming TangJun Yu
Nov 20, 2014·Methods in Molecular Biology·Faisal Reza, Peter M Glazer
Jun 9, 2015·World Journal of Hepatology·Lemonica Koumbi
Jul 29, 2015·World Journal of Gastroenterology : WJG·Peng XiuJie Li
Aug 1, 2014·Acta Pharmaceutica Sinica. B·Jinhong ChangJu-Tao Guo
Oct 28, 2016·Hepatology International·Qiuju Tian, Jidong Jia
Nov 2, 2016·The Journal of Clinical Investigation·Laura RuéEulàlia Martí
Sep 10, 2017·Scientific Reports·Y Vladimir Pabon-MartinezRula Zain

❮ Previous
Next ❯

Citations

Apr 13, 2021·Expert Opinion on Therapeutic Targets·Mohube Betty MaepaPatrick Arbuthnot

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
PCR
electrophoresis
ELISA

Software Mentioned

Blast
RNA structure
SPSS

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
S R XiaoY-B Deng
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Yi-bin DengYesheng Wei
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Yi-bin DengYan-fei Wang
© 2022 Meta ULC. All rights reserved