Antiviral Goes Viral: Harnessing CRISPR/Cas9 to Combat Viruses in Humans

Trends in Microbiology
Jasper Adriaan Soppe, Robert Jan Lebbink

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems are RNA-guided sequence-specific prokaryotic antiviral immune systems. In prokaryotes, small RNA molecules guide Cas effector endonucleases to invading foreign genetic elements in a sequence-dependent manner, resulting in DNA cleavage by the endonuclease upon target binding. A rewired CRISPR/Cas9 system can be used for targeted and precise genome editing in eukaryotic cells. CRISPR/Cas has also been harnessed to target human pathogenic viruses as a potential new antiviral strategy. Here, we review recent CRISPR/Cas9-based approaches to combat specific human viruses in humans and discuss challenges that need to be overcome before CRISPR/Cas9 may be used in the clinic as an antiviral strategy.

Citations

Feb 8, 2018·European Journal of Immunology·Katharina HochheiserMarco J Herold
Apr 21, 2018·The Journal of General Virology·Rutger David LuteijnEmmanuel J H J Wiertz
Apr 29, 2018·Biotechnology Letters·Hasan Mollanoori, Shahram Teimourian
Jan 19, 2018·Journal of Leukocyte Biology·Dominik Hotter, Frank Kirchhoff
Oct 6, 2018·Plant Signaling & Behavior·Manal TashkandiMagdy M Mahfouz
Sep 13, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Anna BellizziHassen S Wollebo
Dec 4, 2019·Genome Biology·Ahmed MahasMagdy Mahfouz
Jul 6, 2020·Microbial Cell Factories·Li LiuXueli Zhang
Jan 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·Xiaohong ChenYuan Ping
Oct 5, 2020·Fundamental & Clinical Pharmacology·Bruna Carolina GonçalvesLígia Carla Faccin-Galhardi
Nov 11, 2020·Scientific Reports·Cathryn M SiegristJoshua L Santarpia
Feb 17, 2021·Pathogens and Global Health·Kyle E WattersGregory D Koblentz
Dec 15, 2020·Trends in Biochemical Sciences·Thi Khanh LePalma Rocchi
Apr 27, 2021·Experimental Biology and Medicine·Arnab BanerjeeBithin K Maji
Dec 15, 2017·ACS Chemical Biology·David B ThompsonGeorge M Church
Aug 21, 2020·Current HIV Research·Edward K MainaRaphael M Lwembe

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