Anxiety-like behavior of mice produced by conditional central expression of the HIV-1 regulatory protein, Tat

Psychopharmacology
Jason J ParisJay P McLaughlin

Abstract

Human immunodeficiency virus (HIV) infection is associated with substantial increases in generalized anxiety. The HIV regulatory protein, transactivator of transcription (Tat), has been implicated in the neuropathogenesis related to HIV-1 infection. However, direct examination of the effect of Tat on behavioral measures of anxiety has not been demonstrated. To identify whether expression of the Tat1-86 protein exerts dose-dependent and persistent anxiety-like effects in a whole animal model, the GT-tg bigenic mouse. GT-tg mice and C57BL/6J controls were administered doxycycline in a dose- (0, 50, 100, or 125 mg/kg, i.p., for 7 days) or duration- (100 mg/kg, i.p., for 0, 1, 3, 5, or 14 days) dependent manner to induce Tat1-86 in brain. Mice were assessed for anxiety-like behavior in an open field, social interaction, or marble burying task 0, 7, and/or 14 days later. Central expression of Tat1-86 protein was verified with Western blot analyses. Doxycycline produced no effects on C57BL/6J controls that lacked the Tat1-86 transgene. Among GT-tg mice, doxycycline (100 mg/kg for 3, 5, or 7 days) significantly increased anxiety-like behavior in all tasks, commensurate with enhanced Western blot labeling of Tat1-86 protein in brain, d...Continue Reading

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Citations

Jan 29, 2014·Journal of Pharmacological and Toxicological Methods·Allan V Kalueff, Michael Nguyen
Nov 26, 2015·Neurobiology of Disease·Gretchen N NeighTanja Jovanovic
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Oct 16, 2021·Journal of Neuroendocrinology·Mohammed F SalahuddinJason J Paris

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