Anxiolytic activity of a supercritical carbon dioxide extract of Souroubea sympetala (Marcgraviaceae)

Phytotherapy Research : PTR
Martha MullallyJ T Arnason

Abstract

The purpose of this work was to develop an extraction technique to yield a betulinic acid-(BA) enriched extract of the traditional anti-anxiety plant Souroubea sympetala Gilg (Marcgraviaceae). Five extraction techniques were compared: supercritical carbon dioxide extraction (SCE), conventional solvent extraction with ethyl acetate (EtOAc), accelerated solvent extraction (ASE), ultrasonic assisted extraction (UAE) and soxhlet extraction (Sox). The EtOAc and SCE extraction methods resulted in BA-enriched extracts, with BA concentrations of 6.78 ± 0.2 and 5.54 ± 0.2 mg/g extract, respectively, as determined by HPLC-APCI-MS. The bioactivity of the BA-enriched extracts was compared in the elevated plus maze (EPM), a validated rodent anxiety behaviour assay. Rats orally administered a 75 mg/kg dose of SCE extract exhibited anxiolysis as compared with vehicle controls, with a 50% increase in the percent time spent in the open arms, a 73% increase in unprotected head dips and a 42% decrease in percent time spent in the closed arms. No significant differences were observed between the SCE and EtOAc extracts for these measures, but the animals dosed with SCE extract had significantly more unprotected head dips than those dosed with the E...Continue Reading

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Citations

Aug 21, 2014·Canadian Journal of Physiology and Pharmacology·Martha MullallyJohn T Arnason
Sep 13, 2017·The AAPS Journal·Rui LiuJohn T Arnason
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aleksandar MasicJohn Arnason
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jan Bachořík, Milan Urban

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