AP-1 and AP-3 mediate sorting of melanosomal and lysosomal membrane proteins into distinct post-Golgi trafficking pathways

Traffic
Björn ChapuyS Höning

Abstract

The adaptor complexes AP-1 and AP-3 are localized to endosomes and/or the trans Golgi network (TGN). Because of limitations in analysing intracellular adaptor function directly, their site of function is a matter of ongoing uncertainty. To overcome this problem and to analyse adaptor sorting at the TGN, we reconstituted vesicle formation from Golgi/TGN-enriched membranes in a novel in vitro budding assay. Melanocytes were metabolically labelled followed by a 19 degrees C temperature block to accumulate newly synthesized proteins in Golgi membranes, which were then enriched by subcellular fractionation and used as donor membranes for vesicle formation in vitro. The incorporation of the melanosomal proteins tyrosinase and tyrosinase-related protein 1 (TRP-1) as well as Lamp-1 and 46 kDa mannose-6-phosphate receptor (MPR46) into Golgi/TGN-derived vesicles was temperature, nucleotide, cytosol, ADP ribosylation factor 1 and adaptor dependent. We show that sorting of TRP-1 and MPR46 was AP-1 dependent, while budding of tyrosinase and Lamp-1 required AP-3. Depletion of clathrin inhibited sorting of all four cargo proteins, suggesting that AP-1 and AP-3 are involved in the formation of distinct types of clathrin-coated vesicles, each o...Continue Reading

References

Mar 15, 1987·International Journal of Cancer. Journal International Du Cancer·D C BennettI R Hart
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J SarasteM G Farquhar
Aug 1, 1986·Journal of Microscopy·K T Tokuyasu
Jun 1, 1984·The Journal of Cell Biology·H J GeuzeK Von Figura
Jun 1, 1993·The Journal of Cell Biology·J KlumpermanH J Geuze
Jan 1, 1996·The Journal of Cell Biology·W StoorvogelH J Geuze
Mar 1, 1997·Biochimica Et Biophysica Acta·G Banting, S Ponnambalam
May 19, 1997·The Journal of Cell Biology·F SimpsonM S Robinson
Aug 8, 1997·The Journal of Biological Chemistry·S HöningK von Figura
May 9, 1998·Science·E C Dell'AngelicaJ S Bonifacino
Feb 20, 1999·The Journal of Biological Chemistry·D ZizioliP Schu
Mar 6, 1999·The Journal of Biological Chemistry·E C Dell'AngelicaJ S Bonifacino
Dec 28, 1999·Annual Review of Cell and Developmental Biology·T Kirchhausen
Nov 10, 2000·Molecular Biology of the Cell·M T DrakeS Kornfeld
May 19, 2001·Science·K S CarrollS R Pfeffer
Jun 2, 2001·Science·R PuertollanoJ S Bonifacino
Jul 14, 2001·Molecular Biology of the Cell·M HuizingR E Boissy
Jan 25, 2002·The Journal of Cell Biology·Andrew A PedenMargaret S Robinson
Apr 16, 2002·Molecular Biology of the Cell·Martin SachseJudith Klumperman
Apr 24, 2002·The Journal of Cell Biology·Ulrike ReinAnne Spang
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Noriyuki NishimuraWilliam E Balch
Jul 10, 2002·Nature Cell Biology·Yunbo GanEnrique Rodriguez-Boulan
Sep 7, 2002·Science·Balraj DorayStuart Kornfeld
Jan 17, 2003·Molecular Biology of the Cell·Satoshi WaguriBernard Hoflack
Nov 18, 2003·Traffic·Guruprasad Ramarao Medigeshi, Peter Schu
Nov 26, 2003·The Journal of Cell Biology·Pradipta GhoshStuart Kornfeld
Jan 7, 2004·Nature Reviews. Molecular Cell Biology·Juan S Bonifacino

❮ Previous
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Citations

Oct 3, 2013·PLoS Genetics·Daniel W SirkisCédric S Asensio
Feb 28, 2009·Genome Research·Curtis HuttenhowerOlga G Troyanskaya
Oct 31, 2009·Journal of the American Society of Nephrology : JASN·Bo ZhouHui Cai
Apr 29, 2011·Molecular Biology of the Cell·Karine LaulagnierJean Gruenberg
Apr 15, 2009·The Journal of Cell Biology·Minoo RaziSharon A Tooze
May 17, 2011·Expert Review of Dermatology·Taisuke Kondo, Vincent J Hearing
Oct 21, 2009·The Journal of Cell Biology·Cédric DelevoyeGraça Raposo
May 20, 2009·The Journal of Cell Biology·Anna V BulankinaStefan Höning
Oct 13, 2010·The International Journal of Biochemistry & Cell Biology·Sanyukta RanaMargot Zoeller
Jun 6, 2009·Current Opinion in Cell Biology·Esteban C Dell'Angelica
Nov 4, 2015·Pigment Cell & Melanoma Research·Sarmistha MahantySubba Rao Gangi Setty
Dec 27, 2008·The Journal of Investigative Dermatology·Hideaki FujitaYoshitaka Tanaka
Apr 24, 2014·Annals of the New York Academy of Sciences·Diego Martinelli, Carlo Dionisi-Vici
Mar 31, 2015·Frontiers in Biology·Rebecca M Fox, Deborah J Andrew
Dec 31, 2016·International Journal of Molecular Sciences·Catherine StaudtMarielle Boonen
Aug 19, 2017·Experimental & Molecular Medicine·Kyu-Han KimEun-Gyung Cho
Nov 2, 2016·Journal of Cell Science·Cinzia Progida, Oddmund Bakke
Apr 9, 2010·Journal of Cell Science·Cinzia ProgidaCecilia Bucci

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