AP-1 and Mitf interact with NFATc1 to stimulate cathepsin K promoter activity in osteoclast precursors

Journal of Cellular Biochemistry
Manhui PangBruce R Troen

Abstract

Cathepsin K (CTSK) is a secreted protease that plays an essential role in osteoclastic bone resorption and osteoporotic bone loss. We have previously shown that activator protein 1 (AP-1) stimulates CTSK promoter activity and that proximal nuclear factor of activated T cells cytoplasmic 1 (NFATc1)-binding sites play a major role in the stimulation of CTSK gene expression by receptor activator of NFκB ligand (RANKL). In the present study, we have extended these observations and further dissected the effects of transcription factors involved in the regulation of CTSK gene expression. Our aim was to investigate the cooperative interplay among transcription factors AP-1, microphthalmia-associated transcription factor (Mitf), and NFATc1, and the consequent regulatory effects on CTSK transcription. Experiments were carried out in RAW 264.7 cells, which can be readily differentiated to osteoclasts upon RANKL stimulation. Our data show that AP-1, Mitf, and NFATc1 are capable of independently stimulating CTSK promoter activity. A combination of any two factors further enhances CTSK promoter activity, with the combination of AP-1 (c-fos/c-jun) and NFATc1 inducing the largest increase. We further identify a synergistic effect when all thr...Continue Reading

References

Nov 13, 1992·Cell·R S JohnsonV Papaioannou
Dec 24, 1992·Nature·Z Q WangE F Wagner
Dec 10, 1991·Biochimica Et Biophysica Acta·P Angel, M Karin
Jan 15, 2004·The Journal of Biological Chemistry·Hiroaki HirotaniNeil A Clipstone
Mar 3, 2004·Drug News & Perspectives·Bruce R Troen
Apr 10, 2004·The Journal of Biological Chemistry·Koichi MatsuoErwin F Wagner
Feb 8, 2005·Biochemical and Biophysical Research Communications·Manhui PangBruce R Troen
Mar 19, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hiroshi Takayanagi
Jul 13, 2006·Annals of the New York Academy of Sciences·Bruce R Troen
Nov 14, 2006·Bone·Masataka Asagiri, Hiroshi Takayanagi
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Qingxiao ZhaoYi-Ping Li
Apr 4, 2007·The Journal of Biological Chemistry·Sudarshana M SharmaMichael C Ostrowski
Sep 28, 2007·Gene·Manhui PangBruce R Troen
Feb 22, 2008·Journal of Cellular Physiology·Tania N CrottiKevin P McHugh
Jan 28, 2009·Calcified Tissue International·Adele L BoskeyMitchell B Schaffler
Jul 7, 2009·FEBS Letters·Insun SongNacksung Kim
Feb 13, 2014·Cell Biochemistry and Function·Kumiko AsaiMasaru Murakami
Dec 10, 2014·Journal of Bone Metabolism·Jung Ha Kim, Nacksung Kim
Mar 4, 2015·Journal of Dental Research·Y XueX Duan

❮ Previous
Next ❯

Citations

May 27, 2020·Biomedizinische Technik. Biomedical Engineering·Claus MosekeAndrea Ewald
Sep 26, 2020·International Journal of Molecular Sciences·Chia-Hsin WuShih-Yu Lee
Oct 12, 2020·Medical Hypotheses·Huaqiang TaoDechun Geng
Nov 14, 2020·International Journal of Molecular Sciences·Hyung Joon KimYong-Deok Kim
Jun 12, 2020·Bone·Jungeun Yu, Ernesto Canalis
Aug 8, 2021·International Journal of Molecular Sciences·Yongguang GaoJingxian Jia
Jul 7, 2021·Journal of Cellular Biochemistry·Purushoth EthirajSakamuri V Reddy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Yu WangDana T Graves
Journal of Cellular Biochemistry
Tomonari KajitaTatsuji Nishihara
© 2022 Meta ULC. All rights reserved