AP-1 and Oct-1 transcription factors down-regulate the expression of the human PIT1/GHF1 gene.

The Journal of Biological Chemistry
M DelhaseE L Hooghe-Peters

Abstract

The pituitary-specific transcription factor Pit-1/GHF-1 is a member of the POU domain family of regulatory proteins. It is involved in the commitment and expansion of the somatotropic cell lineage and activates the transcription of a set of anterior pituitary genes. We have cloned the human PIT1/GHF1 gene and characterized the regulatory mechanisms controlling its promoter activation and regulation. A minimal promoter region (-102 to +15) contains the cis-acting elements that confer to the human PIT1/GHF1 gene a high basal transcriptional activity, the tissue-specific expression, and the autoregulation by Pit-1/GHF-1 protein. The upstream promoter region contains a multiplicity of Pit-1/GHF-1 binding sites that do not show any synergistic interaction with the minimal promoter. The transcriptional activity is negatively regulated by Oct-1 and mediated by an octamer-binding site (OTF). In addition, we have also identified a 12-O-tetradecanoylphorbol-13-acetate-responsive element, which overlaps with a Pit-1/GHF-1 binding site. A mutually exclusive binding of the activator protein-1 (AP-1) and Pit-1/GHF-1 has been observed on this composite site, and AP-1 was shown to down-regulate PIT1/GHF1 transcription.

References

Dec 10, 1991·Biochimica Et Biophysica Acta·P Angel, M Karin
Sep 1, 1990·Molecular and Cellular Biology·B PeersJ A Martial
Jul 28, 1988·Nature·P Sassone-CorsiI M Verma
Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Jan 5, 1995·Biochemical and Biophysical Research Communications·Z D Sharp
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·I BachM G Rosenfeld
Dec 1, 1993·Endocrine Reviews·L E Theill, M Karin
Mar 1, 1994·DNA and Cell Biology·I Berger, Y Shaul
Nov 1, 1993·European Journal of Biochemistry·A J BendallP L Molloy
Oct 1, 1993·Journal of Molecular Endocrinology·M DelhaseE L Hooghe-Peters
Apr 1, 1993·Trends in Endocrinology and Metabolism : TEM·J S ParksR W Pfaffle

❮ Previous
Next ❯

Citations

Jul 11, 2003·Immunology Letters·Elizaveta V PankratovaIvan V Krivega
Aug 22, 2000·European Journal of Biochemistry·M PalazzoloF Blasi
Sep 29, 2011·Human Gene Therapy·Catherine RocheAnne Barlier
Sep 26, 2000·Molecular and Cellular Biology·S LopezS Navarro
May 10, 2013·Doklady. Biochemistry and Biophysics·T N PortsevaE V Pankratova
Apr 9, 2005·Molecular Endocrinology·Brian P Hermann, Leslie L Heckert
Dec 27, 2011·Endocrine-related Cancer·Dario PalmieriMonica Fedele
Dec 17, 2009·Gene·Marina Maria de Jesús Romero-PradoJose Luis Castrillo-Diez
Dec 27, 2005·Molecular and Cellular Endocrinology·Ekaterina BreousUlrich Loos
Oct 5, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yu-Peng WangXiao-Liang Wang
Jun 9, 1999·The Journal of Allergy and Clinical Immunology·V A MordvinovC J Sanderson
May 1, 2008·The Journal of Clinical Endocrinology and Metabolism·F CastinettiT Brue
Jul 4, 2001·The Journal of Biological Chemistry·P T La Celle, R R Polakowska
Nov 9, 2019·The Journal of Clinical Endocrinology and Metabolism·Louise Cheryl Gregory, Mehul Tulsidas Dattani
Sep 8, 2016·Doklady. Biochemistry and Biophysics·T N PortsevaS G Georgieva
Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C S MaloneR Wall
Mar 29, 2003·Immunology Letters·Nadejda N LuchinaElizaveta V Pankratova
Sep 24, 2021·Scientific Reports·Alexander G StepchenkoElizaveta V Pankratova

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Aphasia

Aphasia affects the ability to process language, including formulation and comprehension of language and speech, as well as the ability to read or write. Here is the latest research on aphasia.