AP-2α inhibits hepatocellular carcinoma cell growth and migration

International Journal of Oncology
Wenhuan HuangJian Zhang

Abstract

Transcription factor AP-2α is involved in many types of human cancers, but its role in hepatocellular carcinogenesis is largely unknown. In this study, we found that expression of AP-2α was low in 40% of human hepatocellular cancers compared with adjacent normal tissues by immunohistochemical analysis. Moreover, AP-2α expression was low or absent in hepatocellular cancer cell lines (HepG2, Hep3B, SMMC-7721 and MHHC 97-H). Human liver cancer cell lines SMMC-7721 and Hep3B stably overexpressing AP-2α were established by lentiviral infection and puromycin screening, and the ectopic expression of AP-2α was able to inhibit hepatocellular cancer cell growth and proliferation by cell viability, MTT assay and liquid colony formation in vitro and in vivo. Furthermore, AP-2α overexpression decreased liver cancer cell migration and invasion as assessed by wound healing and Transwell assays, increasing the sensitivity of liver cancer cells to cisplatin analyzed by MTT assays. Also AP-2α overexpression suppressed the sphere formation and renewed the ability of cancer stem cells. Finally, we found that AP-2α is epigenetically modified and modulates the levels of phosphorylated extracellular signal-regulated protein kinase (ERK), β-catenin, p...Continue Reading

References

Jan 1, 1997·Nature Genetics·Y X ZengW S el-Deiry
Nov 17, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J M KarjalainenV M Kosma
May 8, 2001·International Journal of Cancer. Journal International Du Cancer·G J ThomasP M Speight
Mar 21, 2002·Nature Reviews. Cancer·T Soussi, C Béroud
Sep 13, 2002·The Journal of Biological Chemistry·Lisa A McPhersonRonald J Weigel
Apr 23, 2003·Journal of Cancer Research and Clinical Oncology·B Lustig, J Behrens
Dec 4, 2003·Current Opinion in Cell Biology·Jean Paul Thiery
Jun 16, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J PellikainenV-M Kosma
Nov 17, 2005·Oncogene·P R StabachR J Weigel
Mar 15, 2006·The Journal of Biological Chemistry·Narendra WajapeyeeKumaravel Somasundaram
Jul 25, 2006·Critical Reviews in Oncology/hematology·Alejandro FornerJordi Bruix
Mar 3, 2007·International Journal of Cancer. Journal International Du Cancer·Johanna M Pellikainen, Veli-Matti Kosma
Jul 25, 2007·Annual Review of Cell and Developmental Biology·Neethan A LoboMichael F Clarke
Nov 22, 2007·Neoplasia : an International Journal for Oncology Research·Gang ZengSatdarshan P S Monga
Oct 14, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kwan Ho TangKwok Wah Chan
Dec 12, 2012·Oncology Reports·Xiaofeng DingJian Zhang
May 11, 2013·The International Journal of Biochemistry & Cell Biology·Xiaofeng DingJian Zhang
May 18, 2013·The International Journal of Biochemistry & Cell Biology·Miao LiEnjie Luo
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Oct 22, 2014·International Journal of Oncology·Cheng ChenShuanglin Xiang
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal

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Citations

May 4, 2018·Journal of Cellular and Molecular Medicine·Ketao ZhangJianping Liu

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